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Hamster Monoclonal to CD3 (mouse)

145-2C11 (IgG)

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  11-578-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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  1F-578-C100 FITC 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1P-578-C025 PE 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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  1A-578-C025 APC 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1A-578-C100 APC 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  PC-578-C025 PerCP 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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  T9-578-C025 PerCP-Cy™5.5 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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  T7-578-C025 PE-Cy™7 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  T7-578-C100 PE-Cy™7 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins superfamily encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.

Specificity

The Armenian hamster monoclonal antibody 145-2C11 reacts with mouse CD3 (epsilon subunit). This antibody is commonly used as a phenotypic marker for mouse T cells.

Regulatory Status

Immunogen

Mouse BM10-37 cytotoxic T lymphocytes

Species Reactivity:

  • Mouse

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution: 1-2 μg / ml (million cells)
  • Immunoprecipitation
    Recommended dilution: 1-2 μg / 100-500 μg protein in 1 ml cell lysate
  • Immunohistochemistry (frozen sections)
  • Immunocytochemistry
  • Functional Application
    Induction of T cell activation, proliferation or apoptosis (depending on conditions); in vivo T cell depletion
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Leo O, Foo M, Sachs DH, Samelson LE, Bluestone JA: Identification of a monoclonal antibody specific for a murine T3 polypeptide. Proc Natl Acad Sci U S A. 1987 Mar;84(5):1374-8. [Abstract] [Full Text]
  • *Samelson LE, O'Shea JJ, Luong H, Ross P, Urdahl KB, Klausner RD, Bluestone J: T cell antigen receptor phosphorylation induced by an anti-receptor antibody. J Immunol. 1987 Oct 15;139(8):2708-14. [Abstract]
  • *Payer E, Elbe A, Stingl G: Circulating CD3+/T cell receptor V gamma 3+ fetal murine thymocytes home to the skin and give rise to proliferating dendritic epidermal T cells. J Immunol. 1991 Apr 15;146(8):2536-43. [Abstract]
  • *Salvadori S, Gansbacher B, Pizzimenti AM, Zier KS: Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors. J Immunol. 1994 Dec 1;153(11):5176-82. [Abstract]
  • *Jacobs H, Vandeputte D, Tolkamp L, de Vries E, Borst J, Berns A: CD3 components at the surface of pro-T cells can mediate pre-T cell development in vivo. Eur J Immunol. 1994 Apr;24(4):934-9. [Abstract]
  • *Vossen AC, Tibbe GJ, Kroos MJ, van de Winkel JG, Benner R, Savelkoul HF: Fc receptor binding of anti-CD3 monoclonal antibodies is not essential for immunosuppression, but triggers cytokine-related side effects. Eur J Immunol. 1995 Jun;25(6):1492-6. [Abstract]
  • *Henrickson M, Reid J, Bellet JS, Sawchuk SS, Hirsch R: Comparison of in vivo efficacy and mechanism of action of antimurine monoclonal antibodies directed against TCR alpha beta (H57-597) and CD3 (145-2C11). Transplantation. 1995 Oct 27;60(8):828-35. [Abstract]
  • *Kinnaert P, Pradier O, Bournonville B, Habrant C, Goldman M, Van Geertruyden N: Role of CD18-dependent and CD18-independent mechanisms in the increased leukocyte adhesiveness and in the variations of circulating white blood cell populations induced by anti-CD3 monoclonal antibodies. Transpl Int. 1996;9(4):386-91. [Abstract]
  • *Kearse KP: Calnexin associates with monomeric and oligomeric (disulfide-linked) CD3delta proteins in murine T lymphocytes. J Biol Chem. 1998 Jun 5;273(23):14152-7. [Abstract] [Full Text]
  • *Han WR, Murray-Segal LJ, Gershenzon A, Zhang JG, Hodder AN, Pietersz GA, Mottram PL: Idarubicin-145-2C11-F(ab')2 promotes peripheral tolerance and reduces chronic vascular disease in mouse cardiac allografts. Transpl Immunol. 1999 Dec;7(4):207-13. [Abstract]
  • *Tilley SL, Jaradat M, Stapleton C, Dixon D, Hua X, Erikson CJ, McCaskill JG, Chason KD, Liao G, Jania L, Koller BH, Jetten AM: Retinoid-related orphan receptor gamma controls immunoglobulin production and Th1/Th2 cytokine balance in the adaptive immune response to allergen. J Immunol. 2007 Mar 1;178(5):3208-18. [Abstract] [Full Text]
  • *And many other.
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    145-2C11 PE

    Fig. 1. Surface staining of CD3 in murine splenocytes with anti-CD3 (145-2C11) PE.


    145-2C11 PE-Cy7

    Fig. 2. Surface staining of CD3 in murine splenocytes with anti-CD3 (145-2C11) PE-CyTM7.



    145-2C11 PerCP-Cy5-5

    Fig. 3. Surface staining of CD3 in murine splenocytes with anti-CD3 (145-2C11) PerCP-CyTM5.5.



    145-2C11 PerCP

    Fig. 4. Surface staining of CD3 in murine splenocytes with anti-CD3 (145-2C11) PerCP.


    1a-578

    Fig. 5. Surface staining of CD3 in murine splenocytes with anti-CD3 (145-2C11) APC.

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