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categories: Apoptosis (Rodent)

Hamster Monoclonal to Bim (mouse)

Ham151-149 (IgG1 )

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  11-569-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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Bim (Bcl2-interacting mediator) is a pro-apoptotic protein of BH3 domain-only subgroup of the Bcl2 family. It has important roles in initiation of apoptosis in response to many death stimuli. Bim is an important regulator of B and T cell negative selection and is also an essential regulator of T cell apoptosis during termination of an immune response. Bim is constitutively expressed in many cell types but it is maintained in an inactive form through binding to the microtubule-associated dynein motor complex.


The Armenian hamster antibody Ham151-149 reacts with mouse Bim, a 19-24 kDa pro-apoptotic protein (Bcl-2 family) which regulates immunological responses.

Regulatory Status


Mouse BimL (w/o transmembrane region) + Bcl-xL (w/o transmembrane region)-6-his tag, purified from Hi5 cells

Species Reactivity:

  • Mouse

Negative Species:


  • Flow Cytometry
    Recommended dilution:10 μg / ml
    Application note:Cells permeabilized with 0.03% saponin
  • Immunoprecipitation
    Recommended dilution: 1-2 μg /100-500 μg of protein in 1 ml lysate
  • Western Blotting
    Recommended dilution: 1-2 μg / ml Application note:reducing conditions
  • Immunocytochemistry
    Application note:Permeabilized cells
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Hughes P, Bouillet P, Strasser A: Role of Bim and other Bcl-2 family members in autoimmune and degenerative diseases. Curr Dir Autoimmun. 2006;9:74-94. [Abstract]
  • *Snow AL, Oliveira JB, Zheng L, Dale JK, Fleisher TA, Lenardo MJ: Critical role for BIM in T cell receptor restimulation-induced death. Biol Direct. 2008 Aug 20;3:34. [Abstract] [Full Text]
  • *Ludwinski MW, Sun J, Hilliard B, Gong S, Xue F, Carmody RJ, Devirgiliis J, Chen YH: Critical roles of Bim in T cell activation and T cell-mediated autoimmune inflammation in mice. J Clin Invest. 2009 May 1. pii: 37619. doi: 10.1172/JCI37619. [Epub ahead of print] [Abstract] [Full Text]
  • *Yang Y, Zhao Y, Liao W, Yang J, Wu L, Zheng Z, Yu Y, Zhou W, Li L, Feng J, Wang H, Zhu WG: Acetylation of FoxO1 activates Bim expression to induce apoptosis in response to histone deacetylase inhibitor depsipeptide treatment. Neoplasia. 2009 Apr;11(4):313-24. [Abstract] [Full Text]
  • *Wang X, Xing D, Liu L, Chen WR: BimL directly neutralizes Bcl-xL to promote Bax activation during UV-induced apoptosis. FEBS Lett. 2009 May 8. [Epub ahead of print] [Abstract]
  • Product Specific References

  • *Oliver PM, Wang M, Zhu Y, White J, Kappler J, Marrack P: Loss of Bim allows precursor B cell survival but not precursor B cell differentiation in the absence of interleukin 7. J Exp Med. 2004 Nov 1;200(9):1179-87. [Abstract] [Full Text]
  • *Jorgensen TN, McKee A, Wang M, Kushnir E, White J, Refaeli Y, Kappler JW, Marrack P: Bim and Bcl-2 mutually affect the expression of the other in T cells. J Immunol. 2007 Sep 15;179(6):3417-24. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at
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