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Mouse Monoclonal to CD326 / EpCAM

323/A3 (IgG1)

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CD326 / EpCAM (also known as ESA, EGP40, EGP-2, KSA1/4, CO17-1A, GA733-2, MOC31, Ber-EP4) is a 40 kDa transmembrane glycoprotein serving as adhesion molecule in the basolateral membranes in a variety of epithelial cells. CD326 mediates calcium-independent homotypic cell-cell adhesions. CD326 over-expression has been detected in many epithelial tumours and is often associated with bad prognosis. It has been used for diagnostics of (pre-) malignancies at early stages.


The antibody 323/A3 recognizes CD326 / EpCAM, a marker of epithelial lineages, that is over-expressed in many carcinomas.

Regulatory Status


Human breast cancer MCF-7 cells

Species Reactivity:

  • Human

Negative Species:

  • Rat


  • Flow Cytometry
  • Immunoprecipitation
    Recommended dilution: 1-4 μg / ml per 100-500 μg of protein in 1 ml lysate
  • Western Blotting
    Recommended dilution: 1-4 μg/ml
    Positive control: A-431, MCF-7, SW480 cell lines
  • Immunohistochemistry (paraffin sections)
    Recommended dilution: 1-10 μg/ml
    Pretreatment: Tissue section digestion with pepsin (15 min at RT or 10 min at 37°C, 1 mg / ml Tris-HCl, pH 2.0).
    Positive tissue: Breast carcinoma
  • Immunocytochemistry
    Recommended dilution: 1-10 μg/ml
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Edwards DP, Grzyb KT, Dressler LG, Mansel RE, Zava DT, Sledge GW Jr, McGuire WL: Monoclonal antibody identification and characterization of a Mr 43,000 membrane glycoprotein associated with human breast cancer. Cancer Res. 1986 Mar;46(3):1306-17. [Abstract] [Full Text]
  • *Litvinov SV, Velders MP, Bakker HA, Fleuren GJ, Warnaar SO: Ep-CAM: a human epithelial antigen is a homophilic cell-cell adhesion molecule. J Cell Biol. 1994 Apr;125(2):437-46. [Abstract] [Full Text]
  • *Litvinov SV, Bakker HA, Gourevitch MM, Velders MP, Warnaar SO: Evidence for a role of the epithelial glycoprotein 40 (Ep-CAM) in epithelial cell-cell adhesion. Cell Adhes Commun. 1994 Oct;2(5):417-28. [Abstract]
  • *Balzar M, Bakker HA, Briaire-de-Bruijn IH, Fleuren GJ, Warnaar SO, Litvinov SV: Cytoplasmic tail regulates the intercellular adhesion function of the epithelial cell adhesion molecule. Mol Cell Biol. 1998 Aug;18(8):4833-43. [Abstract] [Full Text]
  • *Balzar M, Briaire-de Bruijn IH, Rees-Bakker HA, Prins FA, Helfrich W, de Leij L, Riethmüller G, Alberti S, Warnaar SO, Fleuren GJ, Litvinov SV: Epidermal growth factor-like repeats mediate lateral and reciprocal interactions of Ep-CAM molecules in homophilic adhesions. Mol Cell Biol. 2001 Apr;21(7):2570-80. [Abstract] [Full Text]
  • *Winter MJ, Nagtegaal ID, van Krieken JH, Litvinov SV: The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology. Am J Pathol. 2003 Dec;163(6):2139-48. [Abstract] [Full Text]
  • *Kirman I, Jenkins D, Fowler R, Whelan RL: Naturally occurring antibodies to epithelial cell adhesion molecule (EpCAM). Dig Dis Sci. 2003 Dec;48(12):2306-9. [Abstract]
  • *And many other.
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data

    323-A3 pur

    Fig. 1. Western blotting analysis (non-reducing conditions) of whole cell lysate of MCF-7 human breast adenocarcinoma cell line using anti-CD323 / EpCAM (323/A3.

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