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Mouse Monoclonal to CD66acde

CLB-gran/10 (IgG1)

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Background

The CD66 molecules are 180-200 kDa glycoproteins of carcinoembryonic antigen family. They are present on all blood granulocytes and some tissue macrophages, but are absent from other hematopoietic cells. The expression of CD66 increases significantly on granulocytes upon their activation. CD66a (BGP1, CEACAM1) and CD66d (CGM1, CEACAM3), as well as CEACAM2 and 4, are transmembrane proteins, whereas CD66c (CEAL, NCA, CEACAM6) and CD66e (CEA, CEACAM5), as well as CEACAM7 and 8, are anchored to the plasma membrane by C-terminal glycosylphosphatidylinositol (GPI) lipid moiety.

Specificity

The mouse monoclonal antibody CLB-gran/10 (IH4Fc) detects human CD66acde antigen, a granulocyte marker, especially after cell stimulation. The antibody does not cross-react with normal human peripheral B cells, T cells, monocytes and platelets. Weak reactivity has been observed with malignant cells of pacients with B cell-derived CLL. In immunohistochemistry the antibody reacts with some tissue macrophages and carcinoma-expressed CEA.
HLDA IV; WS Code M38

Regulatory Status

Immunogen

Human granulocytes

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution:2-8 μg/ml
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry (paraffin sections)
    Recommended dilution:20 μg/ml
    Positive tissue:human colon
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Singer BB, Scheffrahn I, Kammerer R, Suttorp N, Ergun S, Slevogt H: Deregulation of the CEACAM expression pattern causes undifferentiated cell growth in human lung adenocarcinoma cells. PLoS One. 2010 Jan 18;5(1):e8747. [Abstract] [Full Text]
  • Product Specific References

  • *Tetteroo PA, Bos MJ, Visser FJ, von dem Borne AE: Neutrophil activation detected by monoclonal antibodies. J Immunol. 1986 May 1;136(9):3427-32. [Abstract]
  • *Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).
  • *Nagel G, Grunert F, Kuijpers TW, Watt SM, Thompson J, Zimmermann W: Genomic organization, splice variants and expression of CGM1, a CD66-related member of the carcinoembryonic antigen gene family. Eur J Biochem. 1993 May 15;214(1):27-35. [Abstract] [Full Text]
  • *Teixeira AM, Fawcett J, Simmons DL, Watt SM: The N-domain of the biliary glycoprotein (BGP) adhesion molecule mediates homotypic binding: domain interactions and epitope analysis of BGPc. Blood. 1994 Jul 1;84(1):211-9. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data


    CLB-gran-10
    Fig. 1.

    Fig. 1. Surface staining of human peripheral blood cells with anti-CD66acde monoclonal antibody (clone CLB-gran/10).

    CLB-gran/10 IHC
    Fig. 2.

    Fig. 2. Immunohistochemistry staining of human colon (paraffin sections) using anti-CD66acde (clone CLB-gran/10). Commercially tested by LifeSpan BioSciences.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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