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Mouse Monoclonal to Ubinuclein 1

UBN1-02 (IgG)

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Background

Ubinuclein 1 (UBN1) is a ubiquitously expressed evolutionarily conserved protein which binds to proliferation-promoting genes that are repressed by formation of senescence-associated heterochromatin foci (SAHF). Ubinuclein 1 associates with various transcription factors and with histone methyltransferase activity, is indispensable for SAHF formation and appears to be a regulator of senescence. Although in most cells ubinuclein is localized to the nucleus, in cells forming tight junctions it is recruited to the cell adhesion complexes, dependently on the cell density.

Specificity

The antibody UBN1-02 recognizes N-terminal part of ubinuclein 1 (UBN1), a widely expressed nuclear and adhesion complex protein. Western blotting analysis reveals UBN1 as a 165 kDa band.

Regulatory Status

Immunogen

Recombinant protein corresponding to amino acids 1-190 of ubinuclein 1.

Species Reactivity:

  • Human
  • Other species Not tested

Negative Species:

Applications:

  • Immunoprecipitation
  • Western Blotting
    Recommended dilution: 1-5 μg/ml
    Positive control: HeLa cell line
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Banumathy G, Somaiah N, Zhang R, Tang Y, Hoffmann J, Andrake M, Ceulemans H, Schultz D, Marmorstein R, Adams PD: Human UBN1 is an ortholog of yeast Hpc2p and has an essential role in the HIRA/ASF1a chromatin-remodeling pathway in senescent cells. Mol Cell Biol. 2009 Feb;29(3):758-70. [Abstract]
  • *Aho S, Lupo J, Coly PA, Sabine A, Castellazzi M, Morand P, Sergeant A, Manet E, Boyer V, Gruffat H: Characterization of the ubinuclein protein as a new member of the nuclear and adhesion complex components (NaCos). Biol Cell. 2008 Sep 30. [Abstract]
  • *Aho S, Buisson M, Pajunen T, Ryoo YW, Giot JF, Gruffat H, Sergeant A, Uitto J: Ubinuclein, a novel nuclear protein interacting with cellular and viral transcription factors. J Cell Biol. 2000 Mar 20;148(6):1165-76. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    ubinuclein
    Fig. 1.


    Fig. 1. Western blotting analysis of ubinuclein in nuclear fraction of HeLa cells. The monoclonal antibody UBN1-01 (lane A, B) detects ubinuclein 1 (165 kDa) and an 180 kDa protein - presumable ubinuclein 2. The antibody UBN1-02 (lane C, D) detects ubinuclein 1. Antibody dilution: A, C - 1 μg/ml; B, D - 5 μg/ml.


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