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Rat Monoclonal to CD8a (mouse)

53-6.7 (IgG2a)

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Product No. Form Quantity Stock Price Datasheet Shop
  11-579-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-579-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  12-579-C100 low endotoxin 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  PB-579-C025 Pacific Blue™ 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  PB-579-C100 Pacific Blue™ 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1F-579-C100 FITC 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  A4-579-C025 Alexa Fluor® 488 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  A4-579-C100 Alexa Fluor® 488 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1P-579-C100 PE 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1A-579-C025 APC 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1A-579-C100 APC 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

The CD8a (CD8 alpha) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.

Specificity

The rat monoclonal antibody 53-6.7 recognizes mouse CD8a (32-34 kDa; alpha chain of the CD8 antigen).

Regulatory Status

Immunogen

Mouse spleen cells

Species Reactivity:

  • Mouse

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution:1.5 μg/ml
  • Immunoprecipitation
  • Immunohistochemistry (paraffin sections)
  • Immunohistochemistry (frozen sections)
    Recommended dilution: 1:1000
    Application note: Formaldehyde fixation is not recommended, acetone fixation is preferred.
  • Immunocytochemistry
  • Functional Application
    Isolation and depletion of CD8+ cells, blocking of cytotoxicity, inhibition of CD8+ T cell proliferation.
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Ledbetter JA, Herzenberg LA: Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 1979;47:63-90. [Abstract]
  • *Ledbetter JA, Rouse RV, Micklem HS, Herzenberg LA: T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J Exp Med. 1980 Aug 1;152(2):280-95. [Abstract] [Full Text]
  • *Takahashi K, Nakata M, Tanaka T, Adachi H, Nakauchi H, Yagita H, Okumura K: CD4 and CD8 regulate interleukin 2 responses of T cells. Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5557-61. [Abstract] [Full Text]
  • *Hata H, Sakaguchi N, Yoshitomi H, Iwakura Y, Sekikawa K, Azuma Y, Kanai C, Moriizumi E, Nomura T, Nakamura T, Sakaguchi S. Distinct contribution of IL-6, TNF-alpha, IL-1, and IL-10 to T cell-mediated spontaneous autoimmune arthritis in mice. J Clin Invest. 2004 Aug;114(4):582-8. [Abstract] [Full Text]
  • *Grabbe S, Varga G, Beissert S, Steinert M, Pendl G, Seeliger S, Bloch W, Peters T, Schwarz T, Sunderk÷tter C, Scharffetter-Kochanek K: Beta2 integrins are required for skin homing of primed T cells but not for priming naive T cells. J Clin Invest. 2002 Jan;109(2):183-92. [Abstract] [Full Text]
  • *Ko SY, Ko HJ, Chang WS, Park SH, Kweon MN, Kang CY: alpha-Galactosylceramide can act as a nasal vaccine adjuvant inducing protective immune responses against viral infection and tumor. J Immunol. 2005 Sep 1;175(5):3309-17. [Abstract] [Full Text]
  • *Kamimura D, Sawa Y, Sato M, Agung E, Hirano T, Murakami M: IL-2 in vivo activities and antitumor efficacy enhanced by an anti-IL-2 mAb. J Immunol. 2006 Jul 1;177(1):306-14. [Abstract] [Full Text]
  • *Bouwer HG, Alberti-Segui C, Montfort MJ, Berkowitz ND, Higgins DE: Directed antigen delivery as a vaccine strategy for an intracellular bacterial pathogen. Proc Natl Acad Sci U S A. 2006 Mar 28;103(13):5102-7. [Abstract] [Full Text]
  • *Mochimaru H, Usui T, Yaguchi T, Nagahama Y, Hasegawa G, Usui Y, Shimmura S, Tsubota K, Amano S, Kawakami Y, Ishida S: Suppression of alkali burn-induced corneal neovascularization by dendritic cell vaccination targeting VEGF receptor 2. Invest Ophthalmol Vis Sci. 2008 May;49(5):2172-7. [Abstract] [Full Text]
  • *And many other.
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    53-6-7 APC


    Fig. 1. Surface staining of murine splenocytes with anti-CD8a (53-6.7) APC.


    53-67 af488


    Fig. 2. Surface staining of murine splenocytes with anti-CD8a (53-6.7) Alexa Fluor® 488.


    53-6-7 PB


    Fig. 3. Surface staining of murine splenocytes with anti-CD8a (53-6.7) Pacific BlueTM.


    53-6-7 PE


    Fig. 4. Surface staining of murine splenocytes with anti-CD8a (53-6.7) PE.


    53-6


    Fig. 5. Surface staining of murine splenocytes with anti-CD8a (53-6.7) FITC.



    53-6-7 pur


    Fig. 6. Surface staining of murine splenocytes with anti-CD8a (53-6.7) purified, DAR-PE.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO┤s term and conditions which are available at www.exbio.cz.
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