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Rat Monoclonal to CD4 (mouse)

GK1.5 (IgG2b)

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Product No. Form Quantity Stock Price Datasheet Shop
  11-580-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-580-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  10-580-C100 azide free 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  12-580-C100 low endotoxin 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1F-580-C100 FITC 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1P-580-C100 PE 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1A-580-C025 APC 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1A-580-C100 APC 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

CD4 is a single chain transmembrane glycoprotein of immunoglobulin supergene family. In its extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). The intracellular region of CD4 associates with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. CD4 binds to MHC class II molecules (by CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (by CDR2-like region in CD4 domain 1) and other ligands, such as IL-16 (by to CD4 domain 3) or L-selectin. CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection. CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).

Specificity

The rat monoclonal antibody GK1.5 reacts with an extracellular epitope of mouse CD4 transmembrane glycoprotein (55 kDa).

Regulatory Status

Immunogen

Mouse CTL clone V4 cells

Species Reactivity:

  • Mouse

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution: 1 μg/million cells
  • Immunoprecipitation
    Recommended dilution: 1-2 μg/100-500 μg of protein in 1 ml lysate
  • Immunohistochemistry (frozen sections)
  • Immunocytochemistry
    Recommended dilution: 1-4 μg/ml
  • Functional Application
    Isolation and depletion of CD4+ T cells, blocking of ligand binding to CD4.
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Dialynas DP, Wilde DB, Marrack P, Pierres A, Wall KA, Havran W, Otten G, Loken MR, Pierres M, Kappler J, et al.: Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with class II MHC antigen-reactivity. Immunol Rev. 1983;74:29-56. [Abstract]
  • *Dialynas DP, Quan ZS, Wall KA, Pierres A, Quintáns J, Loken MR, Pierres M, Fitch FW: Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. J Immunol. 1983 Nov;131(5):2445-51. [Abstract]
  • *Wilde DB, Marrack P, Kappler J, Dialynas DP, Fitch FW: Evidence implicating L3T4 in class II MHC antigen reactivity; monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen-specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines. J Immunol. 1983 Nov;131(5):2178-83. [Abstract]
  • *Wu L, Antica M, Johnson GR, Scollay R, Shortman K: Developmental potential of the earliest precursor cells from the adult mouse thymus. J Exp Med. 1991 Dec 1;174(6):1617-27. [Abstract] [Full Text]
  • *Godfrey DI, Kennedy J, Gately MK, Hakimi J, Hubbard BR, Zlotnik A: IL-12 influences intrathymic T cell development. J Immunol. 1994 Mar 15;152(6):2729-35. [Abstract]
  • *Gavett SH, Chen X, Finkelman F, Wills-Karp M: Depletion of murine CD4+ T lymphocytes prevents antigen-induced airway hyperreactivity and pulmonary eosinophilia. Am J Respir Cell Mol Biol. 1994 Jun;10(6):587-93. [Abstract]
  • *Zheng B, Han S, Kelsoe G: T helper cells in murine germinal centers are antigen-specific emigrants that downregulate Thy-1. J Exp Med. 1996 Sep 1;184(3):1083-91. [Abstract] [Full Text]
  • *Felix NJ, Donermeyer DL, Horvath S, Walters JJ, Gross ML, Suri A, Allen PM: Alloreactive T cells respond specifically to multiple distinct peptide-MHC complexes. Nat Immunol. 2007 Apr;8(4):388-97. [Abstract]
  • *Hu M, Watson D, Zhang GY, Graf N, Wang YM, Sartor M, Howden B, Fletcher J, Alexander SI: Long-term cardiac allograft survival across an MHC mismatch after "pruning" of alloreactive CD4 T cells. J Immunol. 2008 May 15;180(10):6593-603. [Abstract] [Full Text]
  • *Yi H, Zhen Y, Zeng C, Zhang L, Zhao Y: Depleting anti-CD4 monoclonal antibody (GK1.5) treatment: influence on regulatory CD4+CD25+Foxp3+ T cells in mice. Transplantation. 2008 Apr 27;85(8):1167-74. [Abstract]
  • *And many other.
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    GK1-5 PE


    Fig. 1. Surface staining of CD4 in murine splenocytes with anti-CD4 (GK1.5) PE.


    GK1


    Fig. 2. Surface staining of CD4 in murine splenocytes with anti-CD4 (GK1.5) azide free, DAR/APC.


    GK1-5 APC


    Fig. 3. Surface staining of CD4 in murine splenocytes with anti-CD4 (GK1.5) APC.

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