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Mouse Monoclonal to AGR3

AGR3.2 (IgG1)

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  11-561-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-561-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

AGR3 (Anterior Gradient 3) protein, also known as AG3 (hAG-3, HAG3 in human), or BCMP11, is a secreted cytoplasmic protein which is involved in metastasis induction and p53 tumour supressor inhibition. It may serve as molecular marker and potential therapeutic target for hormone-responsive breast tumours. Its Xenopus homolog is associated with anteroposterior fate determination during early development.

Specificity

The antibody AGR3.2 recognizes epitope QYSQALKKV within AGR3 (AG3) protein (19-20 kDa); secreted cytoplasmic protein which can serve as a marker of carcinogenesis.

Regulatory Status

Immunogen

Purified human AGR3 protein

Species Reactivity:

  • Human
  • Other species Not tested

Negative Species:

Applications:

  • Western Blotting
    Recommended dilution: 1 μg/ml
    Positive control: T47D breast cancer cell line
    Negative control: H1299 lung carcinoma cell line
  • Immunohistochemistry (frozen sections)
    Recommended dilution: 1 μg/ml
    Positive tissue: breast cancer
  • Immunocytochemistry
    Recommended dilution: 1 μg/ml
    Positive control: T47D breast cancer cell line
    Negative control: H1299 lung carcinoma cell line
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Persson S, Rosenquist M, Knoblach B, Khosravi-Far R, Sommarin M, Michalak M: Diversity of the protein disulfide isomerase family: identification of breast tumor induced Hag2 and Hag3 as novel members of the protein family. Mol Phylogenet Evol. 2005 Sep;36(3):734-40.
  • *Fletcher GC, Patel S, Tyson K, Adam PJ, Schenker M, Loader JA, Daviet L, Legrain P, Parekh R, Harris AL, Terrett JA: hAG-2 and hAG-3, human homologues of genes involved in differentiation, are associated with oestrogen receptor-positive breast tumours and interact with metastasis gene C4.4a and dystroglycan. Br J Cancer. 2003 Feb 24;88(4):579-85.
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data

    agr wb
    Fig. 1.


    Fig. 1. Western blotting analysis of AGR3 protein by AGR3.1 and AGR3.2 antibody, and of AGR3 and AGR2 protein by AGR3.4 antibody in T47D breast cancer cell line compared to H1229 lung carcinoma cell line.  

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