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Mouse Monoclonal to CD82

C33 (IgG2a)

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Product No. Form Quantity Stock Price Datasheet Shop
  11-559-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-559-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  12-559-C100 low endotoxin 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1B-559-C025 biotin 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1B-559-C100 biotin 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1F-559-T025 FITC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1F-559-T100 FITC 100 tests yes choose region PDF datasheetHTML datasheet buy
  1P-559-T025 PE 25 tests yes choose region PDF datasheetHTML datasheet buy
  1P-559-T100 PE 100 tests yes choose region PDF datasheetHTML datasheet buy
  1A-559-T025 APC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1A-559-T100 APC 100 tests yes choose region PDF datasheetHTML datasheet buy
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Background

CD82 (KAI1), a member of the tetraspanin family, forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules. These complexes influence adhesion, morphology, activation, proliferation and differentiation of B, T and other cells. CD82 regulates cytoskeleton rearrangement and may participate in the turnover of the tetraspanin complex members. Besides in the plasma membrane, CD82 is localized also in endosome/lysosome compartments. Tumour-suppressive roles of CD82 have been demonstrated.

Specificity

The antibody C33 reacts with CD82, a widely expressed cell surface protein of the tetraspanin family. CD82 is also found in endosome/lysosome compartments.

Regulatory Status

Immunogen

C91/PL (human HTLV-1+ T cell line)

Species Reactivity:

  • Human
  • Other not tested

Negative Species:

Applications:

  • Flow Cytometry
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry (paraffin sections)
  • Immunocytochemistry
  • Functional Application
    In human MOLT-4 T-cell line the antibody C33 inhibits syncytium formation induced by coculture with human T-cell leukemia virus type 1 (HTLV-1)-positive human T-cell lines.
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Fukudome K, Furuse M, Imai T, Nishimura M, Takagi S, Hinuma Y, Yoshie O: Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T-cell leukemia virus type 1 (HTLV-1)-induced syncytium formation: altered glycosylation of C33 antigen in HTLV-1-positive T cells. J Virol. 1992 Mar;66(3):1394-401. [Abstract] [Full Text]
  • *Imai T, Yoshie O: C33 antigen and M38 antigen recognized by monoclonal antibodies inhibitory to syncytium formation by human T cell leukemia virus type 1 are both members of the transmembrane 4 superfamily and associate with each other and with CD4 or CD8 in T cells. J Immunol. 1993 Dec 1;151(11):6470-81. [Abstract]
  • *Imai T, Kakizaki M, Nishimura M, Yoshie O: Molecular analyses of the association of CD4 with two members of the transmembrane 4 superfamily, CD81 and CD82. J Immunol. 1995 Aug 1;155(3):1229-39. [Abstract]
  • *Escola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ: Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes. J Biol Chem. 1998 Aug 7;273(32):20121-7. [Abstract] [Full Text]
  • *Ueda T, Ichikawa T, Tamaru J, Mikata A, Akakura K, Akimoto S, Imai T, Yoshie O, Shiraishi T, Yatani R, Ito H, Shimazaki J: Expression of the KAI1 protein in benign prostatic hyperplasia and prostate cancer. Am J Pathol. 1996 Nov;149(5):1435-40. [Abstract]
  • *Schatzlmaier P, Supper V, G÷schl L, Zwirzitz A, Eckerstorfer P, Ellmeier W, Huppa JB, Stockinger H: Rapid multiplex analysis of lipid raft components with single-cell resolution. Sci Signal. 2015 Sep 22;8(395):rs11 [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG2a Isotype Control
  • Example Data

    LS 559


    Fig. 1. Immunohistochemistry staining of human tonsil (paraffin-embedded sections) with anti-CD82 (C33).
    Commercially tested by LifeSpan BioSciences.



    C33 APC


    Fig. 2. Surface staining of CD82 on human peripheral blood cells with anti-CD82 (C33) APC.





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