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Rat Monoclonal to CD9 (mouse)

EM-04 (IgG1)

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Background

CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.

Specificity

The rat monoclonal antibody EM-04 recognizes CD9 antigen, a 24 kDa transmembrane protein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes.

Regulatory Status

Immunogen

Permeabilized murine bone marrow-derived mast cells (BMMC).

Species Reactivity:

  • Mouse

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution: 4 μg/ml
  • Immunoprecipitation
  • Western Blotting
    Recommended dilution: 1 μg/ml
    Application note: non-reducing conditions
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Schmidt C, Künemund V, Wintergerst ES, Schmitz B, Schachner M: CD9 of mouse brain is implicated in neurite outgrowth and cell migration in vitro and is associated with the alpha 6/beta 1 integrin and the neural adhesion molecule L1. J Neurosci Res. 1996 Jan 1;43(1):12-31. [Abstract]
  • *Le Naour F, Rubinstein E, Jasmin C, Prenant M, Boucheix C: Severely reduced female fertility in CD9-deficient mice. Science. 2000 Jan 14;287(5451):319-21. [Abstract]
  • *Liu WM, Cao YJ, Yang YJ, Li J, Hu Z, Duan EK: Tetraspanin CD9 regulates invasion during mouse embryo implantation. J Mol Endocrinol. 2006 Feb;36(1):121-30. [Abstract] [Full Text]
  • *Unternaehrer JJ, Chow A, Pypaert M, Inaba K, Mellman I: The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface. Proc Natl Acad Sci U S A. 2007 Jan 2;104(1):234-9. [Abstract] [Full Text]
  • *Kotha J, Zhang C, Longhurst CM, Lu Y, Jacobs J, Cheng Y, Jennings LK: Functional relevance of tetraspanin CD9 in vascular smooth muscle cell injury phenotypes: a novel target for the prevention of neointimal hyperplasia. Atherosclerosis. 2009 Apr;203(2):377-86. [Abstract]
  • Product Specific References

  • *Athman JJ, Wang Y, McDonald DJ, Boom WH, Harding CV, Wearsch PA: Bacterial Membrane Vesicles Mediate the Release of Mycobacterium tuberculosis Lipoglycans and Lipoproteins from Infected Macrophages. J Immunol. 2015 Aug 1;195(3):1044-53. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    em-04


    Fig. 1. Detection of CD9 in bone marrow-derived mast cell (BMMC) lysates by rat monoclonal EM-04 antibody. 


    EM-04 PE FC


    Fig. 2. Surface staining of CD9 in murine peritoneal fluid cells with anti-CD9 (EM-04) PE.


    EM-04 purified FC


    Fig. 3. Surface staining of CD9 in murine peritoneal fluid cells with anti-CD9 (EM-04) purified, DAR-APC.


    EM-04 APC


    Fig. 4. Surface staining of CD9 in murine peritoneal fluid cells with anti-CD9 (EM-04) APC.


    1B-567


    Fig. 5. Surface staining of CD9 in murine peritoneal fluid cells with anti-CD9 (EM-04) biotin, streptavidin-PE.


    1F-567


    Fig. 6. Surface staining of CD9 in murine peritoneal fluid cells with anti-CD9 (EM-04) FITC.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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