categories: CD and Related Antigens & MHC (Rodent)

Rat Monoclonal to CD9 (mouse)

EM-04 (IgG1)

Product No.	Form	Quantity	Price
11-567-C100	purified	0.1 mg	choose region
1B-567-C100	biotin	0.1 mg	choose region
1F-567-C100	FITC	0.1 mg	choose region
1P-567-C025	PE	0.025 mg	choose region
1P-567-C100	PE	0.1 mg	choose region
1A-567-C025	APC	0.025 mg	choose region
1A-567-C100	APC	0.1 mg	choose region

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Background

CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.

Entrez Gene: 6 58.0 cM

Specificity

The rat monoclonal antibody EM-04 recognizes CD9 antigen, a 24 kDa transmembrane protein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes.

Immunogen

Permeabilized murine bone marrow-derived mast cells (BMMC).

Species Reactivity: Mouse Negative Species:	Applications: Flow Cytometry Recommended dilution: 4 μg/ml Immunoprecipitation Western Blotting Recommended dilution: 1 μg/ml Application note: non-reducing conditions Immunocytochemistry Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Schmidt C, Künemund V, Wintergerst ES, Schmitz B, Schachner M: CD9 of mouse brain is implicated in neurite outgrowth and cell migration in vitro and is associated with the alpha 6/beta 1 integrin and the neural adhesion molecule L1. J Neurosci Res. 1996 Jan 1;43(1):12-31. [Abstract] Le Naour F, Rubinstein E, Jasmin C, Prenant M, Boucheix C: Severely reduced female fertility in CD9-deficient mice. Science. 2000 Jan 14;287(5451):319-21. [Abstract] Liu WM, Cao YJ, Yang YJ, Li J, Hu Z, Duan EK: Tetraspanin CD9 regulates invasion during mouse embryo implantation. J Mol Endocrinol. 2006 Feb;36(1):121-30. [Abstract] [Full Text] Unternaehrer JJ, Chow A, Pypaert M, Inaba K, Mellman I: The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface. Proc Natl Acad Sci U S A. 2007 Jan 2;104(1):234-9. [Abstract] [Full Text] *Kotha J, Zhang C, Longhurst CM, Lu Y, Jacobs J, Cheng Y, Jennings LK: Functional relevance of tetraspanin CD9 in vascular smooth muscle cell injury phenotypes: a novel target for the prevention of neointimal hyperplasia. Atherosclerosis. 2009 Apr;203(2):377-86. [Abstract]

Species Reactivity:

Mouse

Negative Species:

Applications:

Flow Cytometry
Recommended dilution: 4 μg/ml
Immunoprecipitation
Western Blotting
Recommended dilution: 1 μg/ml
Application note: non-reducing conditions
Immunocytochemistry

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Schmidt C, Künemund V, Wintergerst ES, Schmitz B, Schachner M: CD9 of mouse brain is implicated in neurite outgrowth and cell migration in vitro and is associated with the alpha 6/beta 1 integrin and the neural adhesion molecule L1. J Neurosci Res. 1996 Jan 1;43(1):12-31. [Abstract]

*Le Naour F, Rubinstein E, Jasmin C, Prenant M, Boucheix C: Severely reduced female fertility in CD9-deficient mice. Science. 2000 Jan 14;287(5451):319-21. [Abstract]

*Liu WM, Cao YJ, Yang YJ, Li J, Hu Z, Duan EK: Tetraspanin CD9 regulates invasion during mouse embryo implantation. J Mol Endocrinol. 2006 Feb;36(1):121-30. [Abstract] [Full Text]

*Unternaehrer JJ, Chow A, Pypaert M, Inaba K, Mellman I: The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface. Proc Natl Acad Sci U S A. 2007 Jan 2;104(1):234-9. [Abstract] [Full Text]

*Kotha J, Zhang C, Longhurst CM, Lu Y, Jacobs J, Cheng Y, Jennings LK: Functional relevance of tetraspanin CD9 in vascular smooth muscle cell injury phenotypes: a novel target for the prevention of neointimal hyperplasia. Atherosclerosis. 2009 Apr;203(2):377-86. [Abstract]

For research use only. Not for drug, diagnostic or other use.

Example Data

Fig. 1.

Fig. 1. Detection of CD9 in bone marrow-derived mast cell (BMMC) lysates by rat monoclonal EM-04 antibody.

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