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Mouse Monoclonal to CD81

M38 (IgG1)

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Product No. Form Quantity Stock Price Datasheet Shop
  11-558-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-558-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1F-558-T025 FITC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1F-558-T100 FITC 100 tests yes choose region PDF datasheetHTML datasheet buy
  1P-558-T025 PE 25 tests yes choose region PDF datasheetHTML datasheet buy
  1P-558-T100 PE 100 tests yes choose region PDF datasheetHTML datasheet buy
  1A-558-T025 APC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1A-558-T100 APC 100 tests yes choose region PDF datasheetHTML datasheet buy
  T4-558-T025 APC-Cy™7 25 tests yes choose region PDF datasheetHTML datasheet buy
  T4-558-T100 APC-Cy™7 100 tests yes choose region PDF datasheetHTML datasheet buy
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Background

CD81 (TAPA-1), a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T and other cells – e.g. in muscles CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus.

Specificity

The antibody M38 reacts with CD81, a 25 kDa member of the tetraspanin family, expressed on majority of cells.

Regulatory Status

Immunogen

MOLT-4 (human T-ALL cell line)

Species Reactivity:

  • Human
  • Feline (Cat)
  • Rabbit

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution:1 μg/ml
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry (paraffin sections)
  • Immunocytochemistry
  • Functional Application
    In human MOLT-4 T-cell line the antibody M38 inhibits syncytium formation induced by coculture with human T-cell leukemia virus type 1 (HTLV-1)-positive human T-cell lines.
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Fukudome K, Furuse M, Imai T, Nishimura M, Takagi S, Hinuma Y, Yoshie O: Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T-cell leukemia virus type 1 (HTLV-1)-induced syncytium formation: altered glycosylation of C33 antigen in HTLV-1-positive T cells. J Virol. 1992 Mar;66(3):1394-401. [Abstract] [Full Text]
  • *Imai T, Yoshie O: C33 antigen and M38 antigen recognized by monoclonal antibodies inhibitory to syncytium formation by human T cell leukemia virus type 1 are both members of the transmembrane 4 superfamily and associate with each other and with CD4 or CD8 in T cells. J Immunol. 1993 Dec 1;151(11):6470-81. [Abstract]
  • *Imai T, Kakizaki M, Nishimura M, Yoshie O: Molecular analyses of the association of CD4 with two members of the transmembrane 4 superfamily, CD81 and CD82. J Immunol. 1995 Aug 1;155(3):1229-39. [Abstract]
  • *Escola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ: Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes. J Biol Chem. 1998 Aug 7;273(32):20121-7." [Abstract] [Full Text]
  • *Stehlíková O, Chovancová J, Tichý B, Krejčí M, Brychtová Y, Panovská A, Francová Skuhrová H, Burčková K, Borský M, Loja T, Mayer J, Pospíšilová S, Doubek M: Detecting minimal residual disease in patients with chronic lymphocytic leukemia using 8-color flow cytometry protocol in routine hematological practice. Int J Lab Hematol. 2013 Sep 13. doi: 10.1111/ijlh.12149. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

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  • Example Data

    M38 APC-Cy7


    Fig. 1. Surface staining of CD81 in human peripheral blood with anti-CD81 (M38) APC-CyTM7.

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