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Mouse Monoclonal to beta-Catenin

EM-22 (IgG2a)

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Beta-catenin is a multifunctional protein involved both in cell adhesion and in activation of transcription. Calcium-dependent intercellular adhesion transmembrane glycoprotein E-cadherin interacts by its cytoplasmic domain with reciprocally bound alpha, beta and gamma catenin. Beta-catenin links this complex through alpha-actinin to the cytoskeleton. Functional cadherin-catenin system is important for invasiveness of tumour cells. Beta-catenin level in cytoplasm is controlled by glycogen synthase kinase-3 beta. When activity of this kinase is blocked (e.g. by excessive stimulation of Wnt signaling pathway), hypophosphorylated stable form of beta-catenin accumulates in the cytoplasm, translocates to the nucleus and activates transcription of genes including those that are involved in cell cycle control. As a result, cell division and neoplastic transformation are promoted.


The antibody EM-22 reacts with C-terminal part of beta-catenin, an 88 kDa multifunctional protein involved both in cell adhesion and in activation of transcription.

Regulatory Status


Recombinant human beta-catenin

Species Reactivity:

  • Human
  • Mouse
  • Hamster

Negative Species:


  • Flow Cytometry
  • Immunoprecipitation
  • Western Blotting
    Positive control: HT29 human colon adenocarcinoma cell line, FHC human cell line, DLD1 human colon adenocarcinoma cell line
    KW1 mouse cell line C57MG mouse cell line, 3T3 mouse fibroblast cell line
  • Immunocytochemistry
    Positive control: HT29 human colon adenocarcinoma cell line
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Nowak M, Madej JA, Dziegiel P: Expression of E-cadherin, beta-catenin and Ki-67 antigen and their reciprocal relationships in mammary adenocarcinomas in bitches. Folia Histochem Cytobiol. 2007;45(3):233-8. [Abstract]
  • *Jung IM, Chung JK, Kim YA, Kim JE, Heo SC, Ahn YJ, Hwang KT, Kim BG, Lee KL, Kim CW, Kim WH, Chang MS: Epstein-Barr Virus, Beta-Catenin, and E-cadherin in Gastric Carcinomas. J Korean Med Sci. 2007 Oct;22(5):855-61. [Abstract]
  • *Valenta T, Lukas J, Korinek V: HMG box transcription factor TCF-4's interaction with CtBP1 controls the expression of the Wnt target Axin2/Conductin in human embryonic kidney cells. Nucleic Acids Res. 2003 May 1;31(9):2369-80. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data

    EM-22 ICC
    Fig. 1.

    Fig. 1. Immunocytochemistry staining of beta-catenin in human colon adenocarcinoma cell line HT29 using EM-22 antibody (green). Cell nuclei visualized by DAPI (blue). 


    Fig. 2.

    Fig. 2. Western blotting analysis of beta-catenin in murine 3T3 (A), C57 (B) and KW1 (C) cell lines using EM-22 antibody.  


    EM-22 FC
    Fig. 3.

    Fig. 3. Flow cytometry analysis (intracellular) of beta-catenin in human MCF-7 cell line by EM-22-Alexa Fluor® 488 (red) compared to isotype control (blue). 

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