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Rabbit Polyclonal to Grb2

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Grb2 (growth factor receptor-bound protein 2) is a highly conserved adaptor protein composed of central SH2 domain flanked by two terminal SH3 domains. Grb2 mediates coupling of receptor protein tyrosine kinases to a guanine nucleotide exchange factor Sos, thereby enabling the tyrosine kinases to stimulate activity of Ras and hence of MAP kinase pathway. Grb2 also can bind to receptor protein tyrosine phosphatases and affect their signaling.


The polyclonal antibody recognizes Grb2, a highly conserved approximately 26 kDa adaptor protein. The immunogen has 100% identity between man, cow, rat and mouse.

Regulatory Status


Synthetic peptide corresponding to C-terminus of human Grb2 (amino acids 198-217).

Species Reactivity:

  • Human
  • Other species Not tested

Negative Species:


  • Western Blotting
    Recommended dilution: 1 μg/ml
    Positive control: RAMOS human Burkitt lymphoma cell line
    Application note: Reducing conditions.
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Sieh M, Batzer A, Schlessinger J, Weiss A: GRB2 and phospholipase C-gamma 1 associate with a 36- to 38-kilodalton phosphotyrosine protein after T-cell receptor stimulation. Mol Cell Biol. 1994 Jul;14(7):4435-42. [Abstract] [Full Text]
  • *Reif K, Buday L, Downward J, Cantrell DA: SH3 domains of the adapter molecule Grb2 complex with two proteins in T cells: the guanine nucleotide exchange protein Sos and a 75-kDa protein that is a substrate for T cell antigen receptor-activated tyrosine kinases. J Biol Chem. 1994 May 13;269(19):14081-7. [Abstract] [Full Text]
  • *den Hertog J, Hunter T: Tight association of GRB2 with receptor protein-tyrosine phosphatase alpha is mediated by the SH2 and C-terminal SH3 domains. EMBO J. 1996 Jun 17;15(12):3016-27. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data

    Fig. 1.

    Fig. 1. Western Blotting analysis (reducing conditions) of Ramos cell lysate using two batches of rabbit anti-Grb2 polyclonal (A, B).

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