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Rat Monoclonal to CD45 (mouse)

EM-05 (IgG)

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CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.


The antibody EM-05 reacts with mouse CD45 antigen (Leukocyte Common Antigen), a single chain type I transmembrane protein expressed at high level on cells of hematopoietic origin, except erythrocytes and platelets.

Regulatory Status


Murine peripheral blood leukocytes

Species Reactivity:

  • Mouse

Negative Species:


  • Flow Cytometry
    Recommended dilution: 2 μg/ml
    Positive control: murine peripheral blood leukocytes
  • Immunoprecipitation
  • Western Blotting
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Byth KF, Conroy LA, Howlett S, Smith AJ, May J, Alexander DR, Holmes N: CD45-null transgenic mice reveal a positive regulatory role for CD45 in early thymocyte development, in the selection of CD4+CD8+ thymocytes, and B cell maturation. J Exp Med. 1996 Apr 1;183(4):1707-18. [Abstract] [Full Text]
  • *Townsend KP, Vendrame M, Ehrhart J, Faza B, Zeng J, Town T, Tan J: CD45 isoform RB as a molecular target to oppose lipopolysaccharide-induced microglial activation in mice. Neurosci Lett. 2004 May 13;362(1):26-30. [Abstract]
  • *Dawes R, Petrova S, Liu Z, Wraith D, Beverley PC, Tchilian EZ. Combinations of CD45 isoforms are crucial for immune function and disease. J Immunol. 2006 Mar 15;176(6):3417-25. [Abstract] [Full Text]
  • *Desharnais P, Dupéré-Minier G, Hamelin C, Devine P, Bernier J: Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. Apoptosis. 2007 Dec 19. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data


    Fig. 1. Surface staining of C57BL/6 cells and CD45 knock-out cells with anti-CD45 antibodies EM-05 and ALI-4A2.


    Fig. 2. Surface staining of mouse blood lymphocytes with anti-CD45 antibody EM-05-APC.

    EM-05 PerCP FC

    Fig. 3. Surface staining of murine splenocytes with anti-CD45 antibody EM-05 PerCP.

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