Exbio antibodies - Rabbit Polyclonal to GABA B receptor GB1a subunit

Rabbit Polyclonal to GABA B receptor GB1a subunit

	Product No.	Form	Quantity	Stock	Price	Datasheet	Shop
	11-549-C100	purified	0.1 mg		choose region

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Background

GABA B receptor is a G-protein-coupled inhibitory receptor of gamma-aminobutyric acid (GABA), and has important functions in brain by inhibition of adenylyl cyclase and modulation of G-protein-gated Ca²⁺ and K⁺ channels. GABA B receptor is comprised of two subunits, GB1 and GB2 with N-terminal extracellular and C-terminal intracellular domains. The GB1 subunit plays a critical role in ligand binding, whereas the GB2 subunit contains the determinants required for G-protein signaling. Multiple allosteric interactions between the two subunits are required for correct functioning of the receptor. There are two N-terminal splice variants of GB1 subunit, termed GB1a and GB1b; their expression in the central nervous system changes during the ontogenesis and differs between various regions of the brain.

Entrez Gene (mouse): 17 20.4 cM
Entrez Gene (rat): 20p 12

Specificity

The polyclonal antibody recognizes N-terminus of mouse gamma-aminobutyric acid (GABA) B receptor 1a. GB1a apparent MW ~130 kDa.

Regulatory Status

RUO

Immunogen

Synthetic peptide (coupled with THG) derived from aminoacid sequence 100-124 of mouse GABA B receptor 1. The sequence has 100% homology with rat and human.

Species Reactivity: Mouse Rat Other species Not tested Negative Species:	Applications: Western Blotting Immunocytochemistry Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Fritschy JM, Meskenaite V, Weinmann O, Honer M, Benke D, Mohler H: GABAB-receptor splice variants GB1a and GB1b in rat brain: developmental regulation, cellular distribution and extrasynaptic localization. Eur J Neurosci. 1999 Mar;11(3):761-8. [Abstract] Galvez T, Duthey B, Kniazeff J, Blahos J, Rovelli G, Bettler B, Prézeau L, Pin JP: Allosteric interactions between GB1 and GB2 subunits are required for optimal GABA(B) receptor function. EMBO J. 2001 May 1;20(9):2152-9. [Abstract] [Full Text] Ng GY, Bertrand S, Sullivan R, Ethier N, Wang J, Yergey J, Belley M, Trimble L, Bateman K, Alder L, Smith A, McKernan R, Metters K, O'Neill GP, Lacaille JC, Hébert TE: Gamma-aminobutyric acid type B receptors with specific heterodimer composition and postsynaptic actions in hippocampal neurons are targets of anticonvulsant gabapentin action. Mol Pharmacol. 2001 Jan;59(1):144-52. [Abstract] [Full Text] Margeta-Mitrovic M, Jan YN, Jan LY: Ligand-induced signal transduction within heterodimeric GABA(B) receptor. Proc Natl Acad Sci U S A. 2001 Dec 4;98(25):14643-8. [Abstract] [Full Text] Margeta-Mitrovic M, Jan YN, Jan LY: Function of GB1 and GB2 subunits in G protein coupling of GABA(B) receptors. Proc Natl Acad Sci U S A. 2001 Dec 4;98(25):14649-54. [Abstract] [Full Text] Kniazeff J, Galvez T, Labesse G, Pin JP: No ligand binding in the GB2 subunit of the GABA(B) receptor is required for activation and allosteric interaction between the subunits. J Neurosci. 2002 Sep 1;22(17):7352-61. [Abstract] [Full Text]

Species Reactivity:

Mouse
Rat
Other species Not tested

Negative Species:

Applications:

Western Blotting
Immunocytochemistry

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Fritschy JM, Meskenaite V, Weinmann O, Honer M, Benke D, Mohler H: GABAB-receptor splice variants GB1a and GB1b in rat brain: developmental regulation, cellular distribution and extrasynaptic localization. Eur J Neurosci. 1999 Mar;11(3):761-8. [Abstract]

*Galvez T, Duthey B, Kniazeff J, Blahos J, Rovelli G, Bettler B, Prézeau L, Pin JP: Allosteric interactions between GB1 and GB2 subunits are required for optimal GABA(B) receptor function. EMBO J. 2001 May 1;20(9):2152-9. [Abstract] [Full Text]

*Ng GY, Bertrand S, Sullivan R, Ethier N, Wang J, Yergey J, Belley M, Trimble L, Bateman K, Alder L, Smith A, McKernan R, Metters K, O'Neill GP, Lacaille JC, Hébert TE: Gamma-aminobutyric acid type B receptors with specific heterodimer composition and postsynaptic actions in hippocampal neurons are targets of anticonvulsant gabapentin action. Mol Pharmacol. 2001 Jan;59(1):144-52. [Abstract] [Full Text]

*Margeta-Mitrovic M, Jan YN, Jan LY: Ligand-induced signal transduction within heterodimeric GABA(B) receptor. Proc Natl Acad Sci U S A. 2001 Dec 4;98(25):14643-8. [Abstract] [Full Text]

*Margeta-Mitrovic M, Jan YN, Jan LY: Function of GB1 and GB2 subunits in G protein coupling of GABA(B) receptors. Proc Natl Acad Sci U S A. 2001 Dec 4;98(25):14649-54. [Abstract] [Full Text]

*Kniazeff J, Galvez T, Labesse G, Pin JP: No ligand binding in the GB2 subunit of the GABA(B) receptor is required for activation and allosteric interaction between the subunits. J Neurosci. 2002 Sep 1;22(17):7352-61. [Abstract] [Full Text]

For research use only. Not for drug, diagnostic or other use.

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