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Mouse Monoclonal to gamma-tubulin

TU-32 (IgG1)

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The gamma-tubulin (TUBG1; relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01% of tubulin dimer). The gamma-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing gamma-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface.


The antibody TU-32 recognizes an epitope (amino acids 434-449 in human) within C-terminus of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC).

Regulatory Status


human gamma-tubulin peptide EYHAATRPDYISWGTQ, amino acids 434-449

Species Reactivity:

  • Animals
  • Protozoa
  • Plants

Negative Species:


  • Western Blotting
    Recommended dilution:0.5 μg/ml
    Application note: excellent
  • Immunocytochemistry
    Application note: methanol/acetone fixation required
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Wiese C, Zheng Y: Microtubule nucleation: gamma-tubulin and beyond. J Cell Sci. 2006 Oct 15;119(Pt 20):4143-53. [Abstract] [Full Text]
  • *Haren L, Remy MH, Bazin I, Callebaut I, Wright M, Merdes A. NEDD1-dependent recruitment of the gamma-tubulin ring complex to the centrosome is necessary for centriole duplication and spindle assembly. J Cell Biol. 2006 Feb 13;172(4):505-15. [Abstract] [Full Text]
  • Product Specific References

  • *Nováková M, Dráberová E, Schürmann W, Czihak G, Viklický V, Dr-aber P: gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies. Cell Motil Cytoskeleton. 1996;33(1):38-51. [Abstract]
  • *Binarová P., Doležel J., Hause B., Dráber P: Association of g-tubulin with kinetochore/centromeric region of plant chromosomes. Plant J. 14: 751-757, 1998.
  • *Dráberová L, Dráberová E, Surviladze Z, Dráber P, Dráber P. Protein tyrosine kinase p53/p56(lyn) forms complexes with gamma-tubulin in rat basophilic leukemia cells. Int Immunol. 1999 Nov;11(11):1829-39. [Abstract] [Full Text]
  • *Binarová P, Cenklová V, Hause B, Kubátová E, Lysák M, Dolezel J, Bögre L, Dráber P: Nuclear gamma-tubulin during acentriolar plant mitosis. Plant Cell. 2000 Mar;12(3):433-42. [Abstract] [Full Text]
  • *Nohýnková E., Dráber P., Reishig J., Kulda J: Cell-cycle dependent localization of gamma-tubulin in early branching eukaryote, a diplomonad flagellate Giardia intestinalis. Eur. J. Cell Biol. 78: 438-445, 2000.
  • *Linhartová I, Novotná B, Sulimenko V, Dráberová E, Dráber P: Gamma-tubulin in chicken erythrocytes: changes in localization during cell differentiation and characterization of cytoplasmic complexes. Dev Dyn. 2002 Mar;223(2):229-40. [Abstract] [Full Text]
  • *Dráberová E, Macůrek L, Richterová V, Böhm KJ, Dráber P: Monoclonal antibody KN-01 against the heavy chain of kinesin. Folia Biol (Praha). 2002;48(2):77-9.
  • *Sulimenko V, Sulimenko T, Poznanovic S, Nechiporuk-Zloy V, Böhm KJ, Macurek L, Unger E, Dráber P: Association of brain gamma-tubulins with alpha beta-tubulin dimers. Biochem J. 2002 Aug 1;365(Pt 3):889-95. [Abstract] [Full Text]
  • *Libusová L, Sulimenko T, Sulimenko V, Hozák P, Dráber P: gamma-Tubulin in Leishmania: cell cycle-dependent changes in subcellular localization and heterogeneity of its isoforms. Exp Cell Res. 2004 May 1;295(2):375-86. [Abstract]
  • *Katsetos CD, Reddy G, Dráberová E, Smejkalová B, Del Valle L, Ashraf Q, Tadevosyan A, Yelin K, Maraziotis T, Mishra OP, Mörk S, Legido A, Nissanov J, Baas PW, de Chadarévian JP, Dráber P: Altered cellular distribution and subcellular sorting of gamma-tubulin in diffuse astrocytic gliomas and human glioblastoma cell lines. J Neuropathol Exp Neurol. 2006 May;65(5):465-77. [Abstract]
  • *Sulimenko V, Dráberová E, Sulimenko T, Macurek L, Richterová V, Dráber P, Dráber P: Regulation of microtubule formation in activated mast cells by complexes of gamma-tubulin with Fyn and Syk kinases. J Immunol. 2006 Jun 15;176(12):7243-53. [Abstract] [Full Text]
  • *Katsetos CD, Dráberová E, Smejkalová B, Reddy G, Bertrand L, de Chadarévian JP, Legido A, Nissanov J, Baas PW, Dráber P: Class III beta-tubulin and gamma-tubulin are co-expressed and form complexes in human glioblastoma cells. Neurochem Res. 2007 Aug;32(8):1387-98. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data


    Fig. 1. Western blotting analysis of gamma-Tubulin in porcine brain lysate by antibody TU-32.

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