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Mouse Monoclonal to Brg1

BRG-01 (IgG2a)

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Background

Brg1 (Brahma-related gene 1) is an ATPase subunit of SWI2/SNF2-like chromatin-remodeling complexes that enable access of regulatory and effector proteins in transcription, DNA repair and DNA replication. Although Brg1-containing complexes are not essential for general cell survival, they participate in transcriptional regulation of several hundred genes including those involved in interferon and stress response, immune cells differentiation, neurogenesis, cell cycle etc. and is absolutely necessary for mouse embryogenesis. Brg1 is also involved in cell growth arrest, senescence and tumour supression.

Specificity

The antibody BRG-01 recognizes an epitope within C-terminal part of human Brg1, a 205 kDa catalytic subunit of SWI2/SNF2-family chromatin-remodeling complexes.

Regulatory Status

Immunogen

Bacterially produced GST-fused protein representing 242 C-terminal amino acids of human Brg1.

Species Reactivity:

  • Human
  • Other not determined

Negative Species:

Applications:

  • Western Blotting
    Recommended dilution: 10 μg/ml
    Positive control: HEK293 cells
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Ooi L, Belyaev ND, Miyake K, Wood IC, Buckley NJ: BRG1 chromatin remodeling activity is required for efficient chromatin binding by repressor element 1-silencing transcription factor (REST) and facilitates REST-mediated repression. J Biol Chem. 2006 Dec 22;281(51):38974-80. [Abstract] [Full Text]
  • *Eroglu B, Wang G, Tu N, Sun X, Mivechi NF: Critical role of Brg1 member of the SWI/SNF chromatin remodeling complex during neurogenesis and neural crest induction in zebrafish. Dev Dyn. 2006 Oct;235(10):2722-35. [Abstract]
  • *Napolitano MA, Cipollaro M, Cascino A, Melone MA, Giordano A, Galderisi U: Brg1 chromatin remodeling factor is involved in cell growth arrest, apoptosis and senescence of rat mesenchymal stem cells. J Cell Sci. 2007 Aug 15;120(Pt 16):2904-11. [Abstract] [Full Text]
  • *Trotter KW, Archer TK.: The BRG1 transcriptional coregulator. Nucl Recept Signal. 2008 Feb 1;6:e004. [Abstract] [Full Text]
  • *Griffin CT, Brennan J, Magnuson T: The chromatin-remodeling enzyme BRG1 plays an essential role in primitive erythropoiesis and vascular development. Development. 2008 Feb;135(3):493-500. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG2a Isotype Control
  • Example Data


    Brg1
    Fig. 1.



    Fig. 1. Immunostaining of Brg1 by BRG-01 antibody.

    A - Brg1-negative SW13 cells were transfected with Brg1-expression plasmid and analysed by Western blotting for Brg1 expression.

    B - HEK293 cytoplasmic (cyt) and nuclear (nuc) lysates were analysed by Western blotting for Brg1 presence.

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