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Mouse Monoclonal to AHNAK1

EM-09 (IgG1)

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  11-540-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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AHNAK1 (Desmoyokin) is a large (700 kDa) scaffold protein that translocates to the plasma membrane after an increas of extracellular calcium level or upon proteinkinase C activation and regulates extracellular calcium influx mediated by L-type Ca2+ channels. AHNAK1 has been implicated in diverse signal transduction proceses affecting cell differentiation and proliferation. In response to calcium-dependent intercellular contacts AHNAK1 forms multimeric complexes in the plasma membrane, connected with actin and annexin 2/S100A10 assemblies and is thus involved in organization of the plasma membrane architecture. In epithelial cells, AHNAK1 is localized in cytoplasm or is membrane-associated, but in cells of nonepithelial origin AHNAK1 is predominantly nuclear; it has a weak DNA-binding activity and associates with the DNA-ligase IV-XRCC4 complex.


The antibody EM-09 reacts with AHNAK1, a 700 kDa multi-functional adaptor protein expressed mainly in epithelial cell, various types of muscle cells and immune cells.

Regulatory Status


Bacterially expressed fragment of N-terminal domain of human AHNAK1.

Species Reactivity:

  • Human
  • Mouse

Negative Species:


  • Western Blotting
    Positive control: HeLa human cervix carcinoma cell line
  • Immunohistochemistry (frozen sections)
  • Immunocytochemistry
    Positive control: HeLa human cervix carcinoma cell line
    Application note: Permeabilization is required.
  • Immunoprecipitation
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Sussman J, Stokoe D, Ossina N, Shtivelman E: Protein kinase B phosphorylates AHNAK and regulates its subcellular localization. J Cell Biol. 2001 Sep 3;154(5):1019-30. [Abstract] [Full Text]
  • *Gentil BJ, Delphin C, Mbele GO, Deloulme JC, Ferro M, Garin J, Baudier J: The giant protein AHNAK is a specific target for the calcium- and zinc-binding S100B protein: potential implications for Ca2+ homeostasis regulation by S100B. J Biol Chem. 2001 Jun 29;276(26):23253-61. [Abstract] [Full Text]
  • *Haase H, Alvarez J, Petzhold D, Doller A, Behlke J, Erdmann J, Hetzer R, Regitz-Zagrosek V, Vassort G, Morano I: Ahnak is critical for cardiac Ca(V)1.2 calcium channel function and its beta-adrenergic regulation. FASEB J. 2005 Dec;19(14):1969-77. [Abstract] [Full Text]
  • *Matza D, Badou A, Kobayashi KS, Goldsmith-Pestana K, Masuda Y, Komuro A, McMahon-Pratt D, Marchesi VT, Flavell RA: A scaffold protein, AHNAK1, is required for calcium signaling during T cell activation. Immunity. 2008 Jan;28(1):64-74. [Abstract]
  • Product Specific References

  • *Johnson EK, Zhang L, Adams ME, Phillips A, Freitas MA, Froehner SC, Green-Church KB, Montanaro F: Proteomic analysis reveals new cardiac-specific dystrophin-associated proteins. PLoS One. 2012;7(8):e43515. doi: 10.1371/journal.pone.0043515. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data

    Fig. 1.

    Fig. 1. Immunofluorescence staining of AHNAK1 in human primary fibroblasts using anti-AHNAK1 (EM-09; green). Actin filaments were decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

    Fig. 2.

    Fig. 2. Immunofluorescence staining of AHNAK1 in HeLa cell line using anti-AHNAK1 (EM-09; red). Cell nuclei stained with DAPI (blue).

    AHNAK jazyk
    FIG. 3

    Fig. 3. Immunohistochemistry staining (frozen sections) of murine tongue by anti-AHNAK1 antibody (EM-09; red). Actin filaments were decorated by phalloidin (green), cell nuclei stained with DAPI (blue).

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