Mouse Monoclonal to IgG (Fc)
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Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75% of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.
The antibody EM-07 reacts with Fc part of human IgG heavy chain and with isolated Fc fragments.
Fusion protein of human IgG Fc fragment.
- Other not determined
- Western Blotting
Application note: The peroxidase conjugate of this antibody is suitable for detection of human IgG Fc fragments by WB. The antibody EM-07 does not crossreact with IgM.
Application note: The peroxidase conjugate of this antibody is suitable for detection of human IgG Fc fragments by ELISA. The antibody EM-07 does not crossreact with IgM.
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.
*Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975;194:77-95.
*Fuller JM, Keyser JW: Serum immunoglobulins after surgical operation. Clin Chem. 1975 May;21(6):667-71.
*Balogh Z, Merétey K, Falus A, Bozsóky S: Serological abnormalities in juvenile chronic arthritis: a review of 46 cases. Ann Rheum Dis. 1980 Apr;39(2):129-34.
[Abstract] [Full Text]
*Brinkmann V, Heusser CH: T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors. Cell Immunol. 1993 Dec;152(2):323-32.
For research use only. Not for drug, diagnostic or other use.
Mouse IgG1 Isotype Control
IgG κ light chain (1), IgG λ light chain (2) and IgG Fc fragment (3) purified from human serum were analysed by Western blotting with MEM-09 antibody against IgG κ light chain (A) and EM-07 antibody against IgG Fc fragment (B).
ELISA detection of IgG in human serum using HRP conjugated EM-07 antibody against IgG Fc fragment.
Flow cytometry analysis (surface staining) of human peripheral blood using anti-IgG (Fc), clone EM-07, FITC.