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Mouse Monoclonal to Cyclin D1

CD1.1 (IgG1)

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  11-535-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-535-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

Cyclin D1 (PRAD1, Bcl-1) is a cytoplasmic and nuclear protein, which is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Whereas during G1 phase cyclin D1 accumulates in the nucleus, it translocates into the cytoplasm during S phase. Without growth factor-mediated stimulation cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest.

Specificity

The antibody CD1.1 recognizes cyclin D1, an ubiquitously expressed 33 kDa protein that migrates as a 36 kDa band under reducing SDS-PAGE conditions.

Regulatory Status

Immunogen

Purified cyclin D1 protein

Species Reactivity:

  • Human
  • Rat
  • Other not determined

Negative Species:

Applications:

  • Flow Cytometry
    Application note: Membrane permeabilization is required.
  • Immunoprecipitation
    Recommended dilution: 1 μg/ml
  • Western Blotting
    Recommended dilution: 1 μg/ml
  • Immunohistochemistry (paraffin sections)
    Pretreatment: Heat treatment, sodium citrate buffer pH 6.0.
  • Immunohistochemistry (frozen sections)
    Recommended dilution: 2 μg/ml
    Positive tissue: colon
  • Immunocytochemistry
    Recommended dilution: 1 μg/ml
  • ELISA
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Diehl JA, Zindy F, Sherr CJ: Inhibition of cyclin D1 phosphorylation on threonine-286 prevents its rapid degradation via the ubiquitin-proteasome pathway. Genes Dev. 1997 Apr 15;11(8):957-72. [Abstract]
  • *Diehl JA, Cheng M, Roussel MF, Sherr CJ: Glycogen synthase kinase-3beta regulates cyclin D1 proteolysis and subcellular localization. Genes Dev. 1998 Nov 15;12(22):3499-511. [Abstract] [Full Text]
  • *Yang W, Zhang Y, Li Y, Wu Z, Zhu D: Myostatin induces cyclin D1 degradation to cause cell cycle arrest through a phosphatidylinositol 3-kinase/AKT/GSK-3 beta pathway and is antagonized by insulin-like growth factor 1. J Biol Chem. 2007 Feb 9;282(6):3799-808. [Abstract] [Full Text]
  • *Mukherji A, Janbandhu VC, Kumar V: GSK-3beta-dependent destabilization of cyclin D1 mediates replicational stress-induced arrest of cell cycle. FEBS Lett. 2008 Mar 6 [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

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