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Mouse Monoclonal to CD86

BU63 (IgG1)

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Background

CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.

Specificity

The antibody BU63 reacts with CD86 (B7-2), a 70 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
HLDA V; WS Code BP BP072
HLDA V; WS Code A A109
HLDA VI; WS Code BP 95
HLDA VI; WS Code B CD86.9

Regulatory Status

Immunogen

B-lymphoblastoid cell line ARH 77

Species Reactivity:

  • Human
  • Other not determined

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution:5 μg/ml
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry (frozen sections)
  • Functional Application
    The antibody BU63 blocks mixed lymphocyte reaction (MLR) and binding of soluble CTLA-4 (CD152)-mulg fusion protein to CD86 (B7-2).
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Vasilevko V, Ghochikyan A, Holterman MJ, Agadjanyan MG: CD80 (B7-1) and CD86 (B7-2) are functionally equivalent in the initiation and maintenance of CD4+ T-cell proliferation after activation with suboptimal doses of PHA. DNA Cell Biol. 2002 Mar;21(3):137-49. [Abstract]
  • *Yadav D, Judkowski V, Flodstrom-Tullberg M, Sterling L, Redmond WL, Sherman L, Sarvetnick N: B7-2 (CD86) controls the priming of autoreactive CD4 T cell response against pancreatic islets. J Immunol. 2004 Sep 15;173(6):3631-9. [Abstract] [Full Text]
  • *Thomas IJ, Petrich de Marquesini LG, Ravanan R, Smith RM, Guerder S, Flavell RA, Wraith DC, Wen L, Wong FS: CD86 has sustained costimulatory effects on CD8 T cells. J Immunol. 2007 Nov 1;179(9):5936-46. [Abstract]
  • *Eri R, Kodumudi KN, Summerlin DJ, Srinivasan M: Suppression of colon inflammation by CD80 blockade: Evaluation in two murine models of inflammatory bowel disease. Inflamm Bowel Dis. 2008 Jan 9 [Abstract]
  • Product Specific References

  • *Engel P, Gribben JG, Freeman GJ, Zhou LJ, Nozawa Y, Abe M, Nadler LM, Wakasa H, Tedder TF: The B7-2 (B70) costimulatory molecule expressed by monocytes and activated B lymphocytes is the CD86 differentiation antigen. Blood. 1994 Sep 1;84(5):1402-7. [Abstract]
  • *Caux C, Vanbervliet B, Massacrier C, Azuma M, Okumura K, Lanier LL, Banchereau J: B70/B7-2 is identical to CD86 and is the major functional ligand for CD28 expressed on human dendritic cells. J Exp Med. 1994 Nov 1;180(5):1841-7. [Abstract]
  • *Mauri D, Wyss-Coray T, Gallati H, Pichler WJ: Antigen-presenting T cells induce the development of cytotoxic CD4+ T cells. I. Involvement of the CD80-CD28 adhesion molecules. J Immunol. 1995 Jul 1;155(1):118-27. [Abstract]
  • *Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
  • *Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
  • *Giguère JF, Bounou S, Paquette JS, Madrenas J, Tremblay MJ: Insertion of host-derived costimulatory molecules CD80 (B7.1) and CD86 (B7.2) into human immunodeficiency virus type 1 affects the virus life cycle. J Virol. 2004 Jun;78(12):6222-32. [Abstract] [Full Text]
  • *Zhan H, Towler HM, Calder VL: The immunomodulatory role of human conjunctival epithelial cells. Invest Ophthalmol Vis Sci. 2003 Sep;44(9):3906-10. [Abstract] [Full Text]
  • *Kolar GR, Mehta D, Pelayo R, Capra JD: A novel human B cell subpopulation representing the initial germinal center population to express AID. Blood. 2007 Mar 15;109(6):2545-52. [Abstract] [Full Text]
  • *Hovden AO, Karlsen M, Jonsson R, Aarstad HJ, Appel S: Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses. BMC Immunol. 2011 Jan 5;12:2. [Abstract] [Full Text]
  • *Chan A, Baird M, Mercer AA, Fleming SB: Maturation and function of human dendritic cells are inhibited by orf virus-encoded interleukin-10. J Gen Virol. 2006 Nov;87(Pt 11):3177-81. [Abstract] [Full Text]
  • *And many other.
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data

    BU63
    Fig. 1.


    Fig. 1. Surface staining of human peripheral blood cells with anti-CD86 (BU63) PerCP (monocyte gate).

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