categories: CD and Related Antigens (Human), CD Antigens & MHC (Veterinary), CD and Related Antigens & MHC (Rodent)

Mouse Monoclonal to CD79a

HM57 (IgG1)

Product No.	Form	Quantity	Price
11-530-C025	purified	0.025 mg	choose region
11-530-C100	purified	0.1 mg	choose region
1P-530-T025	PE	25 tests	choose region
1P-530-T100	PE	100 tests	choose region
1A-530-T025	APC	25 tests	choose region
1A-530-T100	APC	100 tests	choose region
1Y-530-T025	PE-Dyomics 647	25 tests	choose region
1Y-530-T100	PE-Dyomics 647	100 tests	choose region

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Background

CD79a (Ig alpha, MB1) forms disulfide-linked heterodimer with CD79b (Ig beta). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a is present as an intracellular component. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.

Entrez Gene (human): 19q13.2
Entrez Gene (bovine): 18
Entrez Gene (mouse): 7 5.5 cM

Specificity

The antibody HM57 interacts with CD79a (Ig alpha), a 40-45 kDa subunit of B cell antigen-specific receptor (BCR) and its early developmental forms.
HLDA V; WS Code BC cB018
HLDA VI; WS Code BP 193
HLDA VI; WS Code BP 89
HLDA VI; WS Code B B103
HLDA VI; WS Code B CD79.4

Immunogen

Synthetic peptide corresponding to amino acids 202-216 of human CD79a

Species Reactivity: Human Porcine Mouse Rat Bovine Equine (Horse) Rabbit Guinea pig Opossum Chicken Other not determined Negative Species:	Applications: Flow Cytometry Immunohistochemistry (paraffin sections) Immunohistochemistry (frozen sections) Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Bannish G, Fuentes-Pananá EM, Cambier JC, Pear WS, Monroe J.G.: Ligand-independent signaling functions for the B lymphocyte antigen receptor and their role in positive selection during B lymphopoiesis. J Exp Med. 2001 Dec 3;194(11):1583-96. [Abstract] [Full Text] Pike KA, Iacampo S, Friedmann JE, Ratcliffe M.J.: The cytoplasmic domain of Ig alpha is necessary and sufficient to support efficient early B cell development. J Immunol. 2004 Feb 15;172(4):2210-8. [Abstract] [Full Text] Fuentes-Pananá EM, Bannish G, Shah N, Monroe J.G.: Basal Igalpha/Igbeta signals trigger the coordinated initiation of pre-B cell antigen receptor-dependent processes. J Immunol. 2004 Jul 15;173(2):1000-11. [Abstract] [Full Text] Fuentes-Pananá EM, Bannish G, van der Voort D, King LB, Monroe J.G.: Ig alpha/Ig beta complexes generate signals for B cell development independent of selective plasma membrane compartmentalization. J Immunol. 2005 Feb 1;174(3):1245-52. [Abstract] [Full Text] Fuentes-Pananá EM, Bannish G, Karnell FG, Treml JF, Monroe J.G.: Analysis of the individual contributions of Igalpha (CD79a)- and Igbeta (CD79b)-mediated tonic signaling for bone marrow B cell development and peripheral B cell maturation. J Immunol. 2006 Dec 1;177(11):7913-22. [Abstract] [Full Text] Product Specific References van Noesel CJ, van Lier RA, Cordell JL, Tse AG, van Schijndel GM, de Vries EF, Mason DY, Borst J.: The membrane IgM-associated heterodimer on human B cells is a newly defined B cell antigen that contains the protein product of the mb-1 gene. J Immunol. 1991 Jun 1;146(11):3881-8. [Abstract] Mason DY, Cordell JL, Tse AG, van Dongen JJ, van Noesel CJ, Micklem K, Pulford KA, Valensi F, Comans-Bitter WM, Borst J, et al.: The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells. J Immunol. 1991 Dec 1;147(11):2474-82. [Abstract] Mason DY, van Noesel CJ, Cordell JL, Comans-Bitter WM, Micklem K, Tse AG, van Lier RA, van Dongen J.J.: The B29 and mb-1 polypeptides are differentially expressed during human B cell differentiation. Eur J Immunol. 1992 Oct;22(10):2753-6. [Abstract] Jones M, Cordell JL, Beyers AD, Tse AG, Mason D.Y.: Detection of T and B cells in many animal species using cross-reactive anti-peptide antibodies. J Immunol. 1993 Jun 15;150(12):5429-35. [Abstract] Mason DY, Cordell JL, Brown MH, Borst J, Jones M, Pulford K, Jaffe E, Ralfkiaer E, Dallenbach F, Stein H, et al: CD79a: a novel marker for B-cell neoplasms in routinely processed tissue samples. Blood. 1995 Aug 15;86(4):1453-9. [Abstract] Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995). Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997). *Faldyna M, Samankova P, Leva L, Cerny J, Oujezdska J, Rehakova Z, Sinkora J: Cross-reactive anti-human monoclonal antibodies as a tool for B-cell identification in dogs and pigs. Vet Immunol Immunopathol. 2007 Sep 15;119(1-2):56-62. [Abstract]

Species Reactivity:

Human
Porcine
Mouse
Rat
Bovine
Equine (Horse)
Rabbit
Guinea pig
Opossum
Chicken
Other not determined

Negative Species:

Applications:

Flow Cytometry
Immunohistochemistry (paraffin sections)
Immunohistochemistry (frozen sections)

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Bannish G, Fuentes-Pananá EM, Cambier JC, Pear WS, Monroe J.G.: Ligand-independent signaling functions for the B lymphocyte antigen receptor and their role in positive selection during B lymphopoiesis. J Exp Med. 2001 Dec 3;194(11):1583-96. [Abstract] [Full Text]

*Pike KA, Iacampo S, Friedmann JE, Ratcliffe M.J.: The cytoplasmic domain of Ig alpha is necessary and sufficient to support efficient early B cell development. J Immunol. 2004 Feb 15;172(4):2210-8. [Abstract] [Full Text]

*Fuentes-Pananá EM, Bannish G, Shah N, Monroe J.G.: Basal Igalpha/Igbeta signals trigger the coordinated initiation of pre-B cell antigen receptor-dependent processes. J Immunol. 2004 Jul 15;173(2):1000-11. [Abstract] [Full Text]

*Fuentes-Pananá EM, Bannish G, van der Voort D, King LB, Monroe J.G.: Ig alpha/Ig beta complexes generate signals for B cell development independent of selective plasma membrane compartmentalization. J Immunol. 2005 Feb 1;174(3):1245-52. [Abstract] [Full Text]

*Fuentes-Pananá EM, Bannish G, Karnell FG, Treml JF, Monroe J.G.: Analysis of the individual contributions of Igalpha (CD79a)- and Igbeta (CD79b)-mediated tonic signaling for bone marrow B cell development and peripheral B cell maturation. J Immunol. 2006 Dec 1;177(11):7913-22. [Abstract] [Full Text]

Product Specific References

*van Noesel CJ, van Lier RA, Cordell JL, Tse AG, van Schijndel GM, de Vries EF, Mason DY, Borst J.: The membrane IgM-associated heterodimer on human B cells is a newly defined B cell antigen that contains the protein product of the mb-1 gene. J Immunol. 1991 Jun 1;146(11):3881-8. [Abstract]

*Mason DY, Cordell JL, Tse AG, van Dongen JJ, van Noesel CJ, Micklem K, Pulford KA, Valensi F, Comans-Bitter WM, Borst J, et al.: The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells. J Immunol. 1991 Dec 1;147(11):2474-82. [Abstract]

*Mason DY, van Noesel CJ, Cordell JL, Comans-Bitter WM, Micklem K, Tse AG, van Lier RA, van Dongen J.J.: The B29 and mb-1 polypeptides are differentially expressed during human B cell differentiation. Eur J Immunol. 1992 Oct;22(10):2753-6. [Abstract]

*Jones M, Cordell JL, Beyers AD, Tse AG, Mason D.Y.: Detection of T and B cells in many animal species using cross-reactive anti-peptide antibodies. J Immunol. 1993 Jun 15;150(12):5429-35. [Abstract]

*Mason DY, Cordell JL, Brown MH, Borst J, Jones M, Pulford K, Jaffe E, Ralfkiaer E, Dallenbach F, Stein H, et al: CD79a: a novel marker for B-cell neoplasms in routinely processed tissue samples. Blood. 1995 Aug 15;86(4):1453-9. [Abstract]

*Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).

*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).

*Faldyna M, Samankova P, Leva L, Cerny J, Oujezdska J, Rehakova Z, Sinkora J: Cross-reactive anti-human monoclonal antibodies as a tool for B-cell identification in dogs and pigs. Vet Immunol Immunopathol. 2007 Sep 15;119(1-2):56-62. [Abstract]

For research use only. Not for drug, diagnostic or other use.

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Example Data

Fig. 1.

Fig. 1. Intracellular staining of CD79a with anti-CD79a (HM57) PE (gated on leukemic blast cells) in a patient with childhood B-precursor ALL.

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