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Mouse Monoclonal to LST1

LST1/02 (IgG1)

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  11-528-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-528-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

LST1 (leukocyte-specific transcript, also known as B144) is expressed in cells of myeloid/erythroid lineage (monocytes, granulocytes, dendritic cells, platelets, erythrocytes). At least 14 alternatively spliced variants (LST1/A – LST1/N) can be detected; some of them (LST1/A, B, C, G, I, K) are transmembrane cell surface-exposed forms, the other are soluble. LST1 induces production of long, thin filopodia in dendritic cells, has an inhibitory effect on lymphocyte proliferation and may have an immunomodulatory role. LST1/A is an 11 kDa transmembrane adaptor present in membrane rafts and forms spontaneously covalent homodimers. Its intracellular domain contains two tyrosine motifs, one of them being an ITIM very similar to such motifs in Siglec.

Specificity

The antibody LST1/02 reacts with an extracellular epitope of LST1, an approximately 6-11 kDa protein expressed as various transmembrane or soluble forms. LST1 is found predominantly on monocytes and dendritic cells. It migrates on SDS PAGE gels as approximately 25-28 kDa molecule.

Regulatory Status

Immunogen

KLH-fused peptide corresponding to amino acids SSEGPDLRGRDKRGT of human LST1

Species Reactivity:

  • Human
  • Other not determined

Negative Species:

Applications:

  • Immunoprecipitation
    Positive control: RAJI human Burkitt lymphoma cell line
  • Western Blotting
    Application note:
    It migrates on SDS PAGE gels as approximately 25-28 kDa molecule.
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Rollinger-Holzinger I, Eibl B, Pauly M, Griesser U, Hentges F, Auer B, Pall G, Schratzberger P, Niederwieser D, Weiss EH, Zwierzina H: LST1: a gene with extensive alternative splicing and immunomodulatory function. J Immunol. 2000 Mar 15;164(6):3169-76. [Abstract] [Full Text]
  • *Yu X, Weissman SM: Characterization of the promoter of human leukocyte-specific transcript 1. A small gene with a complex pattern of alternative transcripts. J Biol Chem. 2000 Nov 3;275(44):34597-608. [Abstract] [Full Text]
  • *Raghunathan A, Sivakamasundari R, Wolenski J, Poddar R, Weissman SM: Functional analysis of B144/LST1: a gene in the tumor necrosis factor cluster that induces formation of long filopodia in eukaryotic cells. Exp Cell Res. 2001 Aug 15;268(2):230-44. [Abstract]
  • *Mulcahy H, O'Rourke KP, Adams C, Molloy MG, O'Gara F: LST1 and NCR3 expression in autoimmune inflammation and in response to IFN-gamma, LPS and microbial infection. Immunogenetics. 2006 Jan;57(12):893-903. [Abstract]
  • Product Specific References

  • *Draber P, Stepanek O, Hrdinka M, Drobek A, Chmatal L, Mala L, Ormsby T, Angelisova P, Horejsi V, Brdicka T: LST1/A is a myeloid leukocyte-specific transmembrane adaptor protein recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to the plasma membrane. J Biol Chem. 2012 Jun 29;287(27):22812-21 [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data


    LST
    Fig. 1.


    Fig. 1. Western Blotting analysis (non-reducing conditions) of LST1 in whole cell lysate of U937 human Caucasian histiocytic lymphoma cell line.
    Lane 1: immunostaining with Isotype mouse IgG1 control (PPV-06; cat. no. 11-457-C100)
    Lane 2-3: immunostaining with anti-human LST1 (LST1/02), two independent batches.
    Note: It migrates on SDS PAGE gels as approximately 25-28 kDa molecule.

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