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Mouse Monoclonal to Clathrin heavy chain

BF-06 (IgM)

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Clathrin is a submembrane protein that polymerizes into coat-like lattices, which results in membrane invagination. The basic oligomers are composed of three clathrin heavy chain (180 kDa) and three light chain (30 kDa) subunits and the process of polymerization is dynamically regulated by the light chains. Interaction of clathrin with the plasma membrane is mediated by adaptor proteins (AP1-4) specific for different cellular compartments. Another proteins, such as endophilin, epsin and amphiphysin are involved in membrane invagination and clathrin rearrangements. Finally, dynamin functions at the fission stage of clathrin-mediated endocytosis.


The antibody BF-06 recognizes clathrin heavy chain, an ubiquitously expressed 180 kDa protein involved in receptor-mediated endocytosis.

Regulatory Status

Species Reactivity:

  • Human
  • Porcine
  • Mouse
  • Rat
  • Bovine
  • Other not determined

Negative Species:


  • Flow Cytometry
    Recommended dilution: 2-5 μg/ml
    Positive control: human blood leukocytes
  • Immunoprecipitation
  • Western Blotting
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Smith CJ, Pearse BM: Clathrin: anatomy of a coat protein. Trends Cell Biol. 1999 Sep;9(9):335-8. [Abstract]
  • *Szymkiewicz I, Shupliakov O, Dikic I: Cargo- and compartment-selective endocytic scaffold proteins. Biochem J. 2004 Oct 1;383(Pt 1):1-11. [Abstract] [Full Text]
  • *Wilbur JD, Hwang PK, Brodsky FM: New faces of the familiar clathrin lattice. Traffic. 2005 Apr;6(4):346-50. [Abstract]
  • Product Specific References

  • *Macurek L, Zikova M, Draberova E, Draber P: Monoclonal antibody BF-06 against the heavy chain of clathrin. Folia Biol (Praha). 2003;49(6):238-40. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgM Isotype Control
  • Example Data

    BF-06 Hu fib
    Fig. 1.

    Fig. 1. Immunofluorescence staining of clathrin in human primary fibroblasts using anti-clathrin (BF-06; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

    BF-06 HeLa
    Fig. 2.

    Fig. 2. Immunofluorescence staining of clathrin in human HeLa cell line using anti-clathrin (BF-06; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

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