Mouse Monoclonal to SHIP-1
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(SH2 domain containing inositol phosphatase-1) is a 5´inositol phosphatase that regulates cell responses in lymphocytes and myeloid cells by hydrolyzing the second messenger PI(3,4,5) trisphosphate. SHIP-1 is recruited upon engagement of both inhibitory and activatory receptors, such as FcgammaRIIB, Fcgamma RIII, FcepsilonRI or cytokine and growth factor receptors, and supresses PI3K-dependent signaling, down-regulates cell migration and invasion of transformed cells and phagocytosis. SHIP-1 also serves as a scaffold for the recruitment of other proteins to the plasma membrane.
The antibody SHIP-01 reacts with SHIP-1, a phosphoinositide phosphatase largely confined to hematopoietic cells. Multiple forms of SHIP-1 have been reported with molecular weights of 110, 125, 130, 135 and 145 kDa.
Peptide coresponding to a sequence within N-terminal domain of Human SHIP-1.
- Other not determined
- Flow Cytometry
Recommended dilution: 2-5 μg/ml
Positive control: human blood leukocytes
- Western Blotting
Positive control: RAMOS human cell line
Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Boil for 6 min.
Application note: Reducing conditions.
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.
*Ai J, Maturu A, Johnson W, Wang Y, Marsh CB, Tridandapani S: The inositol phosphatase SHIP-2 down-regulates FcgammaR-mediated phagocytosis in murine macrophages independently of SHIP-1. Blood. 2006 Jan 15;107(2):813-20. [Abstract] [Full Text]
*Tarasenko T, Kole HK, Chi AW, Mentink-Kane MM, Wynn TA, Bolland S: T cell-specific deletion of the inositol phosphatase SHIP reveals its role in regulating Th1/Th2 and cytotoxic responses. Proc Natl Acad Sci U S A. 2007 Jul 3;104(27):11382-7. [Abstract] [Full Text]
*Xing W, Hamaguchi M: Effects of SHIP-1 on MMP2 secretion and invasion of SR3Y1 cells. J Genet Genomics. 2007 Apr;34(4):285-93. [Abstract]
*Harris SJ, Parry RV, Westwick J, Ward SG: Phosphoinositide lipid phosphatases: natural regulators of phosphoinositide
3-kinase signaling in T lymphocytes. J Biol Chem. 2008 Feb 1;283(5):2465-9. [Abstract]
For research use only. Not for drug, diagnostic or other use.
Mouse IgG2a Isotype Control
Fig. 1. Western Blotting
Western Blotting analysis (reducing conditions) of human SHIP-1 in whole cell lysate of THP-1 human acute monocytic leukemia cell line.Lane 1:
immunostaining with Isotype mouse IgG1 control (PPV-04; 11-458-C100) Lane 2,3:
immunostaining with anti-human SHIP-1 (SHIP-01
immunostaining with anti-human SHIP-1 (SHIP-02; cat. no. 11-525-C100)