categories: Signaling of Small GTPases, Signaling of Small GTPases (Human)

Mouse Monoclonal to Sos

SOS-01 (IgG1)

	Product No.	Form	Quantity	Stock	Price	Datasheet	Shop
	11-522-C025	purified	0.025 mg		choose region
	11-522-C100	purified	0.1 mg		choose region

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Background

The guanine nucleotide exchange factor Sos (Son-of-sevenless) is a complex multidomain protein that activates the small GTPase Ras (H-Ras, K-Ras, N-Ras, but not functionally distinct R-Ras) in response to receptor tyrosine kinase stimulation. Nucleotide exchange activity of Sos is stimulated by allosteric Ras binding. By another (separable) guanine exchange factor domain domain Sos modulates activity of Rac/Rho GTPases. Sos thus integrates signals that affect both gene expression and cytoskeletal reorganization; the Sos-mediated Ras-activation and Rac activation differ in composition and stability of the formed complex.

Entrez Gene (human): 2p22-p21

Specificity

The antibody SOS-01 reacts with human Sos, an ubiquitously expressed 150 kDa intracellular protein.

Immunogen

Peptide corresponding to amino acids THPSMHRDGPPLLENAHSS of human Sos protein.

Species Reactivity: Human Mouse Other not determined Negative Species:	Applications: Western Blotting Recommended dilution: 1 μg/ml Positive control: HeLa human cervix carcinoma cell line Application note: Reducing conditions. Immunocytochemistry Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Tian X, Feig LA: Basis for signaling specificity difference between Sos and Ras-GRF guanine nucleotide exchange factors. J Biol Chem. 2001 Dec 14;276(50):47248-56. [Abstract] [Full Text] Innocenti M, Tenca P, Frittoli E, Faretta M, Tocchetti A, Di Fiore PP, Scita G.: Mechanisms through which Sos-1 coordinates the activation of Ras and Rac. J Cell Biol. 2002 Jan 7;156(1):125-36. [Abstract] [Full Text] Silver SJ, Chen F, Doyon L, Zink AW, Rebay I: New class of Son-of-sevenless (Sos) alleles highlights the complexities of Sos function. Genesis. 2004 Aug;39(4):263-72. [Abstract] Freedman TS, Sondermann H, Friedland GD, Kortemme T, Bar-Sagi D, Marqusee S, Kuriyan J.: A Ras-induced conformational switch in the Ras activator Son of sevenless. Proc Natl Acad Sci U S A. 2006 Nov 7;103(45):16692-7. [Abstract] [Full Text] *Yang L, Bashaw G.J.: Son of sevenless directly links the Robo receptor to rac activation to control axon repulsion at the midline. Neuron. 2006 Nov 22;52(4):595-607. [Abstract]

Species Reactivity:

Human
Mouse
Other not determined

Negative Species:

Applications:

Western Blotting
Recommended dilution: 1 μg/ml
Positive control: HeLa human cervix carcinoma cell line
Application note: Reducing conditions.
Immunocytochemistry

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Tian X, Feig LA: Basis for signaling specificity difference between Sos and Ras-GRF guanine nucleotide exchange factors. J Biol Chem. 2001 Dec 14;276(50):47248-56. [Abstract] [Full Text]

*Innocenti M, Tenca P, Frittoli E, Faretta M, Tocchetti A, Di Fiore PP, Scita G.: Mechanisms through which Sos-1 coordinates the activation of Ras and Rac. J Cell Biol. 2002 Jan 7;156(1):125-36. [Abstract] [Full Text]

*Silver SJ, Chen F, Doyon L, Zink AW, Rebay I: New class of Son-of-sevenless (Sos) alleles highlights the complexities of Sos function. Genesis. 2004 Aug;39(4):263-72. [Abstract]

*Freedman TS, Sondermann H, Friedland GD, Kortemme T, Bar-Sagi D, Marqusee S, Kuriyan J.: A Ras-induced conformational switch in the Ras activator Son of sevenless. Proc Natl Acad Sci U S A. 2006 Nov 7;103(45):16692-7. [Abstract] [Full Text]

*Yang L, Bashaw G.J.: Son of sevenless directly links the Robo receptor to rac activation to control axon repulsion at the midline. Neuron. 2006 Nov 22;52(4):595-607. [Abstract]

For research use only. Not for drug, diagnostic or other use.

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Mouse IgG1 Isotype Control

Example Data

Fig. 1.

Fig. 2.

Fig. 3.

Fig. 1. Western Blotting analysis (reducing conditions) of human Sos using anti-Sos (SOS-01).
Lane 1: K562 human Caucasian chronic myeloid leukemia cell line
Lane 2: RAJI human Burkitt lymphoma cell line

Fig. 2. Immunofluorescence staining of Sos in human primary fibroblasts using anti-Sos (SOS-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

Fig. 3. Immunofluorescence staining of Sos in murine transformed fibroblasts using anti-Sos (SOS-01; red). Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue).

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