Mouse Monoclonal to Sos
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The guanine nucleotide exchange factor Sos
(Son-of-sevenless) is a complex multidomain protein that activates the small GTPase Ras (H-Ras, K-Ras, N-Ras, but not functionally distinct R-Ras) in response to receptor tyrosine kinase stimulation. Nucleotide exchange activity of Sos is stimulated by allosteric Ras binding. By another (separable) guanine exchange factor domain domain Sos modulates activity of Rac/Rho GTPases. Sos thus integrates signals that affect both gene expression and cytoskeletal reorganization; the Sos-mediated Ras-activation and Rac activation differ in composition and stability of the formed complex.
The antibody SOS-01 reacts with human Sos, an ubiquitously expressed 150 kDa intracellular protein.
Peptide corresponding to amino acids THPSMHRDGPPLLENAHSS of human Sos protein.
- Other not determined
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.
*Tian X, Feig LA: Basis for signaling specificity difference between Sos and Ras-GRF guanine nucleotide exchange factors. J Biol Chem. 2001 Dec 14;276(50):47248-56. [Abstract] [Full Text]
*Innocenti M, Tenca P, Frittoli E, Faretta M, Tocchetti A, Di Fiore PP, Scita G.: Mechanisms through which Sos-1 coordinates the activation of Ras and Rac. J Cell Biol. 2002 Jan 7;156(1):125-36. [Abstract] [Full Text]
*Silver SJ, Chen F, Doyon L, Zink AW, Rebay I: New class of Son-of-sevenless (Sos) alleles highlights the complexities of Sos function. Genesis. 2004 Aug;39(4):263-72.
*Freedman TS, Sondermann H, Friedland GD, Kortemme T, Bar-Sagi D, Marqusee S, Kuriyan J.: A Ras-induced conformational switch in the Ras activator Son of sevenless. Proc Natl Acad Sci U S A. 2006 Nov 7;103(45):16692-7. [Abstract] [Full Text]
*Yang L, Bashaw GJ: Son of sevenless directly links the Robo receptor to rac activation to control axon repulsion at the midline. Neuron. 2006 Nov 22;52(4):595-607.
For research use only. Not for drug, diagnostic or other use.
Mouse IgG1 Isotype Control
Fig. 1. Western Blotting analysis (reducing conditions) of human Sos using anti-Sos (SOS-01).
Lane 1: K562 human Caucasian chronic myeloid leukemia cell line
Lane 2: RAJI human Burkitt lymphoma cell line
Fig. 2. Immunofluorescence staining of Sos in human primary fibroblasts using anti-Sos (SOS-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
Fig. 3. Immunofluorescence staining of Sos in murine transformed fibroblasts using anti-Sos (SOS-01; red). Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue).