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Mouse Monoclonal to PKAc

6D2.1 (IgG1)

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  11-523-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-523-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

Protein kinase A (PKA, cAMP-dependent protein kinase) is a key element of a ubiquitous signaling pathway important in the cell cycle, cellular communication, memory formation and behavior. PKA is composed of two catalytic (PKAc; Protein Kinase A catalytic subunit) and two regulatory subunits (PKAr). Upon binding cAMP, the complex dissociates to PKAr dimer and two activated PKAc ser/thr protein kinase catalytic monomers. The released PKAc can translocate into the nucleus and exert a regulatory role in the activation of multiple nuclear hormone receptors. However, PKAc-mediated activation of tonicity-dependent gene expression is cAMP independent. Humans express three types of PKAc subunit – PKAc alpha is present in most human tissues, PKAc beta and gamma are tissue-specific, the later is found in testes.

Specificity

The antibody 6D2.1 strongly reacts with human proteinkinase A catalytic (PKAc) alpha subunit, and weakly with PKAc gamma subunit (both around 40 kDa). The recognized epitope of PKAc alpha is identical between man, sheep, pig, ox and dog.

Regulatory Status

Immunogen

Peptide corresponding to amino acids ESPAQNTAHLDQFERIK of human proteinkinase A c alpha (PKAc alpha).

Species Reactivity:

  • Human
  • Other not determined

Negative Species:

Applications:

  • Western Blotting
    Recommended dilution: 0.5-1 μg/ml
    Positive control: HeLa human cervix carcinoma cell line
    Application note: To detect PKAc gamma, use a more concentrated lysate from a tissue expressing this subunit (testis).
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Beebe SJ, Oyen O, Sandberg M, Frøysa A, Hansson V, Jahnsen T: Molecular cloning of a tissue-specific protein kinase (C gamma) from human testis--representing a third isoform for the catalytic subunit of cAMP-dependent protein kinase. Mol Endocrinol. 1990 Mar;4(3):465-75. [Abstract]
  • *Kiger JA Jr, Eklund JL, Younger SH, O'Kane CJ: Transgenic inhibitors identify two roles for protein kinase A in Drosophila development. Genetics. 1999 May;152(1):281-90. [Abstract] [Full Text]
  • *Doucas V, Shi Y, Miyamoto S, West A, Verma I, Evans RM: Cytoplasmic catalytic subunit of protein kinase A mediates cross-repression by NF-kappa B and the glucocorticoid receptor. Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):11893-8. [Abstract] [Full Text]
  • *Ferraris JD, Persaud P, Williams CK, Chen Y, Burg MB: cAMP-independent role of PKA in tonicity-induced transactivation of tonicity-responsive enhancer/ osmotic response element-binding protein. Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16800-5. [Abstract] [Full Text]
  • *Komagiri Y, Kitamura N: Comparison of effects of PKA catalytic subunit on I(h) and calcium channel currents in rat dorsal root ganglion cells. Biomed Res. 2007 Aug;28(4):177-89. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data


    6D2.1
    Fig. 1.







    Fig. 1. Dot Blot analysis of GST and GST-fusion proteins using anti-PKAc (6D2.1) and anti-GST (S-tag-05; cat. no. 11-477-C100).
    The total amount of material spotted on the nitrocellulose membrane is 5 ng/spot.
    Lane 1: GST-Akt1
    Lane 2: GST-Akt2
    Lane 3: GST-Akt3
    Lane 4: GST-PKAc alpha
    Lane 5: GST-PKAc beta
    Lane 6: GST-PKAc gamma
    Lane 7: GST-MEK 1
    Lane 8: GST


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