Mouse Monoclonal to PKAc
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Protein kinase A (PKA, cAMP-dependent protein kinase) is a key element of a ubiquitous signaling pathway important in the cell cycle, cellular communication, memory formation and behavior. PKA is composed of two catalytic (PKAc
; Protein Kinase A catalytic subunit) and two regulatory subunits (PKAr). Upon binding cAMP, the complex dissociates to PKAr dimer and two activated PKAc ser/thr protein kinase catalytic monomers. The released PKAc can translocate into the nucleus and exert a regulatory role in the activation of multiple nuclear hormone receptors. However, PKAc-mediated activation of tonicity-dependent gene expression is cAMP independent. Humans express three types of PKAc subunit – PKAc alpha is present in most human tissues, PKAc beta and gamma are tissue-specific, the later is found in testes.
The antibody 6D2.1 strongly reacts with human proteinkinase A catalytic (PKAc) alpha subunit, and weakly with PKAc gamma subunit (both around 40 kDa). The recognized epitope of PKAc alpha is identical between man, sheep, pig, ox and dog.
Peptide corresponding to amino acids ESPAQNTAHLDQFERIK of human proteinkinase A c alpha (PKAc alpha).
- Other not determined
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.
*Beebe SJ, Oyen O, Sandberg M, Frøysa A, Hansson V, Jahnsen T: Molecular cloning of a tissue-specific protein kinase (C gamma) from human testis--representing a third isoform for the catalytic subunit of cAMP-dependent
protein kinase. Mol Endocrinol. 1990 Mar;4(3):465-75.
*Kiger JA Jr, Eklund JL, Younger SH, O'Kane CJ: Transgenic inhibitors identify two roles for protein kinase A in Drosophila
development. Genetics. 1999 May;152(1):281-90.
[Abstract] [Full Text]
*Doucas V, Shi Y, Miyamoto S, West A, Verma I, Evans RM: Cytoplasmic catalytic subunit of protein kinase A mediates cross-repression by NF-kappa B and the glucocorticoid receptor. Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):11893-8.
[Abstract] [Full Text]
*Ferraris JD, Persaud P, Williams CK, Chen Y, Burg MB: cAMP-independent role of PKA in tonicity-induced transactivation of tonicity-responsive enhancer/ osmotic response element-binding protein. Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16800-5. [Abstract] [Full Text]
*Komagiri Y, Kitamura N: Comparison of effects of PKA catalytic subunit on I(h) and calcium channel currents in rat dorsal root ganglion cells. Biomed Res. 2007 Aug;28(4):177-89.
For research use only. Not for drug, diagnostic or other use.
Dot Blot analysis of GST and GST-fusion proteins using anti-PKAc (6D2.1
) and anti-GST (S-tag-05; cat. no. 11-477-C100).
The total amount of material spotted on the nitrocellulose membrane is 5 ng/spot.Lane 1:
GST-PKAc alphaLane 5:
GST-PKAc betaLane 6:
GST-PKAc gammaLane 7:
GST-MEK 1Lane 8: