categories: CD and Related Antigens (Human), CD Antigens & MHC (Veterinary)

Mouse Monoclonal to CD3

UCHT1 (IgG1)

Product No.	Form	Quantity	Price
11-514-C025	purified	0.025 mg	choose region
11-514-C100	purified	0.1 mg	choose region
12-514-C100	low endotoxin	0.1 mg	choose region
PB-514-T025	Pacific Blue™	25 tests	choose region
PB-514-T100	Pacific Blue™	100 tests	choose region
1F-514-T025	FITC	25 tests	choose region
1F-514-T100	FITC	100 tests	choose region
PO-514-T025	Pacific Orange™	25 tests	choose region
PO-514-T100	Pacific Orange™	100 tests	choose region
1P-514-T025	PE	25 tests	choose region
1P-514-T100	PE	100 tests	choose region
1A-514-T025	APC	25 tests	choose region
1A-514-T100	APC	100 tests	choose region
T8-514-T025	PE-Cy™5	25 tests	choose region
T8-514-T100	PE-Cy™5	100 tests	choose region
PC-514-T025	PerCP	25 tests	choose region
PC-514-T100	PerCP	100 tests	choose region
T9-514-T025	PerCP-Cy™5.5	25 tests	choose region
T9-514-T100	PerCP-Cy™5.5	100 tests	choose region

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Background

CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Entrez Gene: 11q23

Specificity

The antibody UCHT1 recognizes the CD3 antigen of the TCR/CD3 complex on mature human T cells. The UCHT1 antibody reacts with the epsilon chain of the CD3 complex.
HLDA I; WS Code T 3
HLDA III; WS Code T 126
HLDA III; WS Code T 471
HLDA VI; WS Code T 6T-CD3.1

Immunogen

human thymocytes followed by Sezary T cells

Species Reactivity: Human Non-Human Primates Negative Species:	Applications: Flow Cytometry Application note: The epitope for UCHT1, CD3 is resistant to fixation. For purposes where pre-fixed cells are stained, this antibody is recommended. Immunoprecipitation Immunohistochemistry (frozen sections) Application note: The epitope for UCHT1, CD3 is resistant to fixation. For purposes where pre-fixed cells are stained, this antibody is recommended. Functional Application The immobilized UCHT1 antibody initiates a signaling pathway resulting in T cell activation and proliferation. Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Huang Y, Wange RL: T cell receptor signaling: beyond complex complexes. J Biol Chem. 2004 Jul 9;279(28):28827-30. [Abstract] [Full Text] Kuhns MS, Davis MM, Garcia KC: Deconstructing the form and function of the TCR/CD3 complex. Immunity. 2006 Feb;24(2):133-9. [Abstract] Alarcón B, Swamy M, van Santen HM, Schamel WW: T-cell antigen-receptor stoichiometry: pre-clustering for sensitivity. EMBO Rep. 2006 May;7(5):490-5. [Abstract] [Full Text] Product Specific References Garson JA, Beverley PC, Coakham HB, Harper EI: Monoclonal antibodies against human T lymphocytes label Purkinje neurones of many species. Nature. 1982 Jul 22;298(5872):375-7. [Abstract] Leukocyte Typing III., McMichael A. J. et al (Eds.), Oxford University Press (1987). Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997). Barclay, Brown et al.: The Leukocyte Antigen FactsBook, 2nd edition, Harcourt Brace & Company, London, (1997). Siegers GM, Swamy M, Fernández-Malavé E, Minguet S, Rathmann S, Guardo AC, Pérez-Flores V, Regueiro JR, Alarcón B, Fisch P, Schamel WW: Different composition of the human and the mouse gammadelta T cell receptor explains different phenotypes of CD3gamma and CD3delta immunodeficiencies. J Exp Med. 2007 Oct 29;204(11):2537-44. [Abstract] [Full Text] Fisch P, Malkovsky M, Braakman E, Sturm E, Bolhuis RL, Prieve A, Sosman JA, Lam VA, Sondel PM: Gamma/delta T cell clones and natural killer cell clones mediate distinct patterns of non-major histocompatibility complex-restricted cytolysis. J Exp Med. 1990 May 1;171(5):1567-79. [Abstract] [Full Text] Demedts IK, Brusselle GG, Vermaelen KY, Pauwels RA: Identification and characterization of human pulmonary dendritic cells. Am J Respir Cell Mol Biol. 2005 Mar;32(3):177-84. [Abstract] [Full Text] Lin CW, Liu TY, Chen SU, Wang KT, Medeiros LJ, Hsu SM: CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features. Blood. 2005 Nov 15;106(10):3567-74. [Abstract] [Full Text] le Gouvello S, Manceau V, Sobel A: Serine 16 of stathmin as a cytosolic target for Ca2+/calmodulin-dependent kinase II after CD2 triggering of human T lymphocytes. J Immunol. 1998 Aug 1;161(3):1113-22. [Abstract] [Full Text] Torres PS, Alcover A, Zapata DA, Arnaud J, Pacheco A, Martín-Fernández JM, Villasevil EM, Sanal O, Regueiro JR: TCR dynamics in human mature T lymphocytes lacking CD3 gamma. J Immunol. 2003 Jun 15;170(12):5947-55. [Abstract] [Full Text] Arnett KL, Harrison SC, Wiley DC: Crystal structure of a human CD3-epsilon/delta dimer in complex with a UCHT1 single-chain antibody fragment. Proc Natl Acad Sci U S A. 2004 Nov 16;101(46):16268-73. [Abstract] [Full Text] Rieux-Laucat F, Hivroz C, Lim A, Mateo V, Pellier I, Selz F, Fischer A, Le Deist F: Inherited and somatic CD3zeta mutations in a patient with T-cell deficiency. N Engl J Med. 2006 May 4;354(18):1913-21. [Abstract] [Full Text] And many other.

Species Reactivity:

Human
Non-Human Primates

Negative Species:

Applications:

Flow Cytometry
Application note:
The epitope for UCHT1, CD3 is resistant to fixation. For purposes where pre-fixed cells are stained, this antibody is recommended.
Immunoprecipitation
Immunohistochemistry (frozen sections)
Application note: The epitope for UCHT1, CD3 is resistant to fixation. For purposes where pre-fixed cells are stained, this antibody is recommended.
Functional Application
The immobilized UCHT1 antibody initiates a signaling pathway resulting in T cell activation and proliferation.

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Huang Y, Wange RL: T cell receptor signaling: beyond complex complexes. J Biol Chem. 2004 Jul 9;279(28):28827-30. [Abstract] [Full Text]

*Kuhns MS, Davis MM, Garcia KC: Deconstructing the form and function of the TCR/CD3 complex. Immunity. 2006 Feb;24(2):133-9. [Abstract]

*Alarcón B, Swamy M, van Santen HM, Schamel WW: T-cell antigen-receptor stoichiometry: pre-clustering for sensitivity. EMBO Rep. 2006 May;7(5):490-5. [Abstract] [Full Text]

Product Specific References

*Garson JA, Beverley PC, Coakham HB, Harper EI: Monoclonal antibodies against human T lymphocytes label Purkinje neurones of many species. Nature. 1982 Jul 22;298(5872):375-7. [Abstract]

*Leukocyte Typing III., McMichael A. J. et al (Eds.), Oxford University Press (1987).

*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).

*Barclay, Brown et al.: The Leukocyte Antigen FactsBook, 2nd edition, Harcourt Brace & Company, London, (1997).

*Siegers GM, Swamy M, Fernández-Malavé E, Minguet S, Rathmann S, Guardo AC, Pérez-Flores V, Regueiro JR, Alarcón B, Fisch P, Schamel WW: Different composition of the human and the mouse gammadelta T cell receptor explains different phenotypes of CD3gamma and CD3delta immunodeficiencies. J Exp Med. 2007 Oct 29;204(11):2537-44. [Abstract] [Full Text]

*Fisch P, Malkovsky M, Braakman E, Sturm E, Bolhuis RL, Prieve A, Sosman JA, Lam VA, Sondel PM: Gamma/delta T cell clones and natural killer cell clones mediate distinct patterns of non-major histocompatibility complex-restricted cytolysis. J Exp Med. 1990 May 1;171(5):1567-79. [Abstract] [Full Text]

*Demedts IK, Brusselle GG, Vermaelen KY, Pauwels RA: Identification and characterization of human pulmonary dendritic cells. Am J Respir Cell Mol Biol. 2005 Mar;32(3):177-84. [Abstract] [Full Text]

*Lin CW, Liu TY, Chen SU, Wang KT, Medeiros LJ, Hsu SM: CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features. Blood. 2005 Nov 15;106(10):3567-74. [Abstract] [Full Text]

*le Gouvello S, Manceau V, Sobel A: Serine 16 of stathmin as a cytosolic target for Ca2+/calmodulin-dependent kinase II after CD2 triggering of human T lymphocytes. J Immunol. 1998 Aug 1;161(3):1113-22. [Abstract] [Full Text]

*Torres PS, Alcover A, Zapata DA, Arnaud J, Pacheco A, Martín-Fernández JM, Villasevil EM, Sanal O, Regueiro JR: TCR dynamics in human mature T lymphocytes lacking CD3 gamma. J Immunol. 2003 Jun 15;170(12):5947-55. [Abstract] [Full Text]

*Arnett KL, Harrison SC, Wiley DC: Crystal structure of a human CD3-epsilon/delta dimer in complex with a UCHT1 single-chain antibody fragment. Proc Natl Acad Sci U S A. 2004 Nov 16;101(46):16268-73. [Abstract] [Full Text]

*Rieux-Laucat F, Hivroz C, Lim A, Mateo V, Pellier I, Selz F, Fischer A, Le Deist F: Inherited and somatic CD3zeta mutations in a patient with T-cell deficiency. N Engl J Med. 2006 May 4;354(18):1913-21. [Abstract] [Full Text]

*And many other.

For research use only. Not for drug, diagnostic or other use.

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Mouse IgG1 Isotype Control

Example Data

Fig. 1.

Fig. 1. Surface staining of human peripheral blood cells with anti-human CD3 (UCHT1) Pacific Blue^TM. Cells in the lymphocyte gate were used for analysis.

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