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Mouse Monoclonal to Lambda light chains

4C2 (IgG1)

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Product No. Form Quantity Stock Price Datasheet Shop
  11-505-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-505-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1F-505-T025 FITC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1F-505-T100 FITC 100 tests yes choose region PDF datasheetHTML datasheet buy
  1P-505-T025 PE 25 tests yes choose region PDF datasheetHTML datasheet buy
  1P-505-T100 PE 100 tests yes choose region PDF datasheetHTML datasheet buy
  1A-505-T025 APC 25 tests yes choose region PDF datasheetHTML datasheet buy
  1A-505-T100 APC 100 tests yes choose region PDF datasheetHTML datasheet buy
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Background

Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda; both having a molecular weight of 22.5kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.

Specificity

The antibody 4C2 reacts with lambda light chains (22.5 kDa) of human immunoglobulin.

Regulatory Status

Species Reactivity:

  • Human

Negative Species:

  • Hamster
  • Sheep
  • Goat
  • Rabbit
  • Guinea pig

Applications:

  • Flow Cytometry
    Recommended dilution:2-8 μg/ml
    Positive control: RAMOS human cell line
  • Immunohistochemistry (paraffin sections)
  • Immunohistochemistry (frozen sections)
  • ELISA
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975;194:77-95. [Abstract]
  • Product Specific References

  • *Os A, Bürgler S, Ribes AP, Funderud A, Wang D, Thompson KM, Tjønnfjord GE, Bogen B, Munthe LA: Chronic lymphocytic leukemia cells are activated and proliferate in response to specific T helper cells. Cell Rep. 2013 Aug 15;4(3):566-77. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data

    kappa lambda


    Fig. 1. B lymphocytes (CD19+) in a dot-plot Lambda PE vs. Kappa FITC. Kappa light chain detected by A8B5 antibody and lambda light chain by 4C2 antibody, CD19 by LT19 antibody. 


    4C2 FITC


    Fig. 2. Surface staining of human peripheral blood with anti-human lambda light chain (4C2) FITC.


    4C2 APC


    Fig. 3. Surface staining of human peripheral blood with anti-human lambda light chain (4C2) APC.


    4C2 purified


    Fig. 4. Surface staining of human peripheral blood with anti-human lambda light chain (4C2) purified, GAM-APC.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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