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Mouse Monoclonal to Kappa light chains

A8B5 (IgG1)

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Background

Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda; both having a molecular weight of 22.5kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.

Specificity

The antibody A8B5 reacts with kappa light chains (22.5 kDa) of immunoglobulins.

Regulatory Status

Species Reactivity:

  • Human

Negative Species:

  • Hamster
  • Sheep
  • Goat
  • Rabbit
  • Guinea pig

Applications:

  • Flow Cytometry
    Positive control: Daudi human cell line
  • Immunohistochemistry (paraffin sections)
  • Immunohistochemistry (frozen sections)
  • ELISA
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975;194:77-95. [Abstract]
  • Product Specific References

  • *Konig A, Wennemuth G, Soyer HP, Hoffmann R, Happle R, Krause W: Vulvar amyloidosis mimicking giant condylomata acuminata in a patient with multiple myeloma. Eur J Dermatol. 1999 Jan-Feb;9(1):29-31. [Abstract]
  • *Avilés-Salas A, Chanona-Vilchis JG, Candelaria-Hernández MG, Peña-Torres Mde L, Cuéllar-Mendoza ME: Presentación inicial de un linfoma plasmablástico como tumor ovárico. Ginecol Obstet Mex. 2012 Jun;80(6):430-4. [Abstract]
  • *Diaz de Domingo NB, Lardo MM, Romero Artaza J, Merelli A, Carbia CD, Sánchez Avalos JC: Aplicacion de tecnicas inmunocitoquimicas para el diagnostico de neoplasias hematologicas. Medicina (B Aires). 1999;59(1):11-6. [Abstract]
  • *Scheeren FA, Diehl SA, Smit LA, Beaumont T, Naspetti M, Bende RJ, Blom B, Karube K, Ohshima K, van Noesel CJ, Spits H: IL-21 is expressed in Hodgkin lymphoma and activates STAT5: evidence that activated STAT5 is required for Hodgkin lymphomagenesis. Blood. 2008 May 1;111(9):4706-15. doi: 10.1182/blood-2007-08-105643 [Abstract] [Full Text]
  • *Di Bartolo DL, Hyjek E, Keller S, Guasparri I, Deng H, Sun R, Chadburn A, Knowles DM, Cesarman E: Role of defective Oct-2 and OCA-B expression in immunoglobulin production and Kaposi's sarcoma-associated herpesvirus lytic reactivation in primary effusion lymphoma. J Virol. 2009 May;83(9):4308-15. doi: 10.1128/JVI.02196-08. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data

    kappa lambda
    Fig. 1.


    Fig. 1. B lymphocytes (CD19+) in a dot-plot Lambda PE vs. Kappa FITC. Kappa light chain detected by A8B5 antibody and lambda light chain by 4C2 antibody, CD19 by LT19 antibody.  

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