categories: CD and Related Antigens (Human)

Mouse Monoclonal to CD22 / BL-CAM

IS7 (IgG1)

Product No.	Form	Quantity	Price
10-506-C025	azide free	0.025 mg	choose region
10-506-C100	azide free	0.1 mg	choose region
PB-506-T025	Pacific Blue™	25 tests	choose region
PB-506-T100	Pacific Blue™	100 tests	choose region
1F-506-T025	FITC	25 tests	choose region
1F-506-T100	FITC	100 tests	choose region
1P-506-T025	PE	25 tests	choose region
1P-506-T100	PE	100 tests	choose region
1A-506-T025	APC	25 tests	choose region
1A-506-T100	APC	100 tests	choose region
A7-506-T025	Alexa Fluor® 700	25 tests	choose region
A7-506-T100	Alexa Fluor® 700	100 tests	choose region

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Background

CD22, also known as Siglec-2 (sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding alpha2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.

Entrez Gene: 19q13.1

Specificity

The antibody IS7 reacts with CD22 (BL-CAM), a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes; it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes.
HLDA IV; WS Code B 227
HLDA V; WS Code B CD22.8

Immunogen

Reh human cell line

Species Reactivity: Human Negative Species:	Applications: Flow Cytometry Positive control: lysed whole blood Application note: lyse, wash protocol Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Tedder TF, Poe JC, Haas KM: CD22: A Multifunctional Receptor That Regulates B Lymphocyte Survival and Signal Transduction. Adv Immunol. 2005;88:1-50. [Abstract] Tateno H, Li H, Schur MJ, Bovin N, Crocker PR, Wakarchuk WW, Paulson JC: Distinct endocytic mechanisms of CD22 (Siglec-2) and Siglec-F reflect roles in cell signaling and innate immunity. Mol Cell Biol. 2007 Aug;27(16):5699-710. [Abstract] [Full Text] Walker JA, Smith KG: CD22: an inhibitory enigma. Immunology. 2007 Dec 7 [Abstract] Product Specific References Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989). *Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).

Species Reactivity:

Human

Negative Species:

Applications:

Flow Cytometry
Positive control: lysed whole blood
Application note: lyse, wash protocol

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Tedder TF, Poe JC, Haas KM: CD22: A Multifunctional Receptor That Regulates B Lymphocyte Survival and Signal Transduction. Adv Immunol. 2005;88:1-50. [Abstract]

*Tateno H, Li H, Schur MJ, Bovin N, Crocker PR, Wakarchuk WW, Paulson JC: Distinct endocytic mechanisms of CD22 (Siglec-2) and Siglec-F reflect roles in cell signaling and innate immunity. Mol Cell Biol. 2007 Aug;27(16):5699-710. [Abstract] [Full Text]

*Walker JA, Smith KG: CD22: an inhibitory enigma. Immunology. 2007 Dec 7 [Abstract]

Product Specific References

*Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).

*Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).

For research use only. Not for drug, diagnostic or other use.

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