- Flow Cytometry
Recommended dilution:2 μg/ml
- Western Blotting
Positive material: HPB cell line
Negative material: JURKAT human cell line, RAJI human cell line
Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.
Application note: Non-reducing conditions.
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.
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[Abstract] [Full Text]
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