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Mouse Monoclonal to HLA-G

4H84 (IgG1)

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Product No. Form Quantity Stock Price Datasheet Shop
  11-499-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-499-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
  1B-499-C025 biotin 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  1B-499-C100 biotin 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

Specificity

The antibody 4H84 recognizes HLA-G molecule (39 kDa). HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.
This product is for research and in vitro experimental use only. It is not to be used for any other commercial purpose. Use of this product to produce products for sale or for therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the Univesity of California.

Regulatory Status

Immunogen

amino acids 61-83 of HLA-G of human origin

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Western Blotting
  • Immunoprecipitation
  • Immunohistochemistry (paraffin sections)
    Recommended dilution:10 μg/ml
  • Immunocytochemistry
  • ELISA
    Application note: The antibody 4H84 has been tested as the capture antibody in a sandwich ELISA for analysis of heavy chains of all HLA-G isoforms in combination with antibody MEM-G/1 (cat. no. 1B-291-C100).
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Paul P, Rouas-Freiss N, Khalil-Daher I, Moreau P, Riteau B, Le Gal FA, Avril MF, Dausset J, Guillet JG, Carosella ED: HLA-G expression in melanoma: a way for tumor cells to escape from immunosurveillance. Proc Natl Acad Sci U S A. 1998 Apr 14;95(8):4510-5. [Abstract] [Full Text]
  • *Gonen-Gross T, Achdout H, Arnon TI, Gazit R, Stern N, Horejsí V, Goldman-Wohl D, Yagel S, Mandelboim O: The CD85J/leukocyte inhibitory receptor-1 distinguishes between conformed and beta 2-microglobulin-free HLA-G molecules. J Immunol. 2005 Oct 15;175(8):4866-74. [Abstract] [Full Text]
  • *Blaschitz A, Hutter H, Dohr G: HLA Class I protein expression in the human placenta. Early Pregnancy. 2001 Jan;5(1):67-9. [Abstract]
  • *Urosevic M, Kurrer MO, Kamarashev J, Mueller B, Weder W, Burg G, Stahel RA, Dummer R, Trojan A: Human leukocyte antigen G up-regulation in lung cancer associates with high-grade histology, human leukocyte antigen class I loss and interleukin-10 production. Am J Pathol. 2001 Sep;159(3):817-24. [Abstract] [Full Text]
  • *Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, Le Danff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N: Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum Immunol. 2003 Mar;64(3):315-26. [Abstract]
  • *Polakova K, Krcova M, Kuba D, Russ G: Analysis of HLA-G expression in malignant hematopoetic cells from leukemia patients. Leuk Res. 2003 Jul;27(7):643-8. [Abstract]
  • *Polakova K, Kuba D, Russ G: The 4H84 monoclonal antibody detecting beta2m free nonclassical HLA-G molecules also binds to free heavy chains of classical HLA class I antigens present on activated lymphocytes. Hum Immunol. 2004 Feb;65(2):157-62. [Abstract]
  • *Juch H, Blaschitz A, Daxbock C, Rueckert C, Kofler K, Dohr G: A novel sandwich ELISA for alpha1 domain based detection of soluble HLA-G heavy chains. J Immunol Methods. 2005 Dec 20;307(1-2):96-106. [Abstract]
  • *Barrier BF, Kendall BS, Sharpe-Timms KL, Kost ER: Characterization of human leukocyte antigen-G (HLA-G) expression in endometrial adenocarcinoma. Gynecol Oncol. 2006 Oct;103(1):25-30. [Abstract]
  • *And many other.
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data

    WB
    Fig. 1.


    Fig. 1. Immunoprecipitation of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by MEM-G/9 antibody and protein G. HLA-G was detected by 4H84 antibody and goat anti-mouse-HRP conjugate in cell lysate (Lane 1) and in the immunoprecipitate (Lane 2). 


    4H84 biotin WB


    Fig. 2. Western blotting analysis of HLA-G in HLA-G1 transfectants (LCL-HLA-G1) by 4H84 antibody-biotin.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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