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Mouse Monoclonal to STIM1 / GOK

CDN3H4 (IgG1)

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STIM1 (stromal interacting molecule; also known as GOK) acts as a sensor of calcium depletion within the endoplasmic reticulum and transduces the signal to Orai1, the presumptive CRAC channel at the plasma membrane. Following decrease of luminal calcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry of extracellular calcium into the cytoplasm. However, the precise mechanism of STIM1-Orai1 interaction has not been elucidated yet. Many questions also remain to be solved around STIM1 functional distribution. It turns out that STIM1 associates with growing ends of microtubules and is involved in endoplasmic reticulum tubule extension.


The antibody CDN3H4 reacts with human and rodent STIM1, a 84 kDa essential and conserved regulator of store-operated Ca2+ channel function.

Regulatory Status


Synthetized peptide (C-terminal cytoplasmic part of STIM1).

Species Reactivity:

  • Human
  • Mouse
  • Rat

Negative Species:


  • Immunoprecipitation
  • Western Blotting
    Recommended dilution: 1 μg/ml
    Positive control: RBL rat basophilic leukemia cell line
    Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer.
    Application note: Both reducing and non-reducing conditions.
  • Immunohistochemistry (paraffin sections)
    Recommended dilution: 5 μg/ml
  • Immunocytochemistry
    Staining technique: methanol-aceton fixation
    Positive control: HeLa human cervix carcinoma cell line
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Williams RT, Manji SS, Parker NJ, Hancock MS, Van Stekelenburg L, Eid JP, Senior PV, Kazenwadel JS, Shandala T, Saint R, Smith PJ, Dziadek MA: Identification and characterization of the STIM (stromal interaction molecule) gene family: coding for a novel class of transmembrane proteins. Biochem J. 2001 Aug 1;357(Pt 3):673-85. [Abstract] [Full Text]
  • *Soboloff J, Spassova MA, Tang XD, Hewavitharana T, Xu W, Gill DL: Orai1 and STIM reconstitute store-operated calcium channel function. J Biol Chem. 2006 Jul 28;281(30):20661-5. [Abstract] [Full Text]
  • *Peel SE, Liu B, Hall IP: A key role for STIM1 in store operated calcium channel activation in airway smooth muscle. Respir Res. 2006 Sep 20;7:119. [Abstract] [Full Text]
  • *Cai X: Molecular evolution and functional divergence of the Ca2+ sensor protein in store-operated Ca2+ entry: stromal interaction molecule. PLoS ONE. 2007 Jul 11;2(7):e609. [Abstract] [Full Text]
  • *Hewavitharana T, Deng X, Soboloff J, Gill DL: Role of STIM and Orai proteins in the store-operated calcium signaling pathway. Cell Calcium. 2007 Aug;42(2):173-82. [Abstract]
  • *Grigoriev I, Gouveia SM, van der Vaart B, Demmers J, Smyth JT, Honnappa S, Splinter D, Steinmetz MO, Putney JW Jr, Hoogenraad CC, Akhmanova A: STIM1 is a MT-plus-end-tracking protein involved in remodeling of the ER. Curr Biol. 2008 Feb 12;18(3):177-82. [Abstract]
  • *Oh-Hora M, Yamashita M, Hogan PG, Sharma S, Lamperti E, Chung W, Prakriya M, Feske S, Rao A: Dual functions for the endoplasmic reticulum calcium sensors STIM1 and STIM2 in T cell activation and tolerance. Nat Immunol. 2008 Mar 9 [Epub ahead of print] [Abstract]
  • *Feske S: ORAI1 and STIM1 deficiency in human and mice: roles of store-operated Ca2+ entry in the immune system and beyond. Immunol Rev. 2009 Sep;231(1):189-209. [Abstract]
  • Product Specific References

  • *Hájková Z, Bugajev V, Dráberová E, Vinopal S, Dráberová L, Janáček J, Dráber P, Dráber P: STIM1-directed reorganization of microtubules in activated mast cells. J Immunol. 2011 Jan 15;186(2):913-23. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data

    Fig. 1.

    Fig. 1. Immunofluorescence staining of HeLa human cervix carcinoma cell line using anti-STIM1 (CDN3H4; methanol-aceton fixation; detection by Goat anti-mouse IgG1 Alexa Fluor® 598; red). Nuclei were stained with DAPI (blue).

    CDN3H4 WB
    Fig. 2.

    Fig. 2. Western Blotting analysis (non-reducing conditions) of whole cell lysate of RBL rat basophilic leukemia cell line.
    Lane 1: immunostaining with anti-STIM1 (CDN3H4)
    Lane 2: immunostaining with Isotype mouse IgG1 control (PPV-06; cat. no. 11-457-C100)

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