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Mouse Monoclonal to PLSCR1 (rat)

13A6 [TEC-23] (IgG2a)

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Background

PLSCR1 (Phospholipid Scramblase 1) is a multiply palmitoylated endofacial plasma membrane protein containing several SH3 and WW domain binding motives. In the plasma membrane, PLSCR1 plays a role in transbilayer lipid redistributions and signal transduction. Nonpalmitoylated PLSCR1, however, is able to be transported into the nucleus and bind DNA. PLSCR1 potentiates the antiviral activity of interferon and its expression is highly induced by interferons and growth factors.

Specificity

The antibody 13A6 [TEC-23] binds to rat Phospholipid Scramblase 1 (PLSCR1), an 37-49 kDa membrane-associated protein accelerating bidirectional movement of plasma membrane phospholipids during conditions of elevated calcium.

Regulatory Status

Immunogen

BALB/c mice were immunized with pooled lipid rafts isolated from RBL-2H3 cells. Hybridoma cells were obtained after fusion of SP02 mouse myeloma cells with spleen cells of immunized mice using standard procedures.

Species Reactivity:

  • Rat

Negative Species:

Applications:

  • Immunoprecipitation
  • Western Blotting
    Recommended dilution: 1 μg/ml
    Positive control: RBL rat basophilic leukemia cell line
    Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% Triton X-100 in 20 mM Tris/Cl, 100 mM NaCl, 2 mM EDTA, 2 mM Na3Vo4, 10 mM glycerolphosphate, pH 8.0, 1 mM PMSF, 10 μg/ml aprotinin). Incubate 20 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer. Boil for 5 min.
    Application note: Both reducing and non-reducing conditions.
  • Flow Cytometry
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Pastorelli C, Veiga J, Charles N, Voignier E, Moussu H, Monteiro RC, Benhamou M: IgE receptor type I-dependent tyrosine phosphorylation of phospholipid scramblase. J Biol Chem. 2001 Jun 8;276(23):20407-12. Epub 2001 Mar 20. [Abstract] [Full Text]
  • *Silverman RH, Halloum A, Zhou A, Dong B, Al-Zoghaibi F, Kushner D, Zhou Q, Zhao J, Wiedmer T, Sims PJ: Suppression of ovarian carcinoma cell growth in vivo by the interferon-inducible plasma membrane protein, phospholipid scramblase 1. Cancer Res. 2002 Jan 15;62(2):397-402. [Abstract] [Full Text]
  • *Dong B, Zhou Q, Zhao J, Zhou A, Harty RN, Bose S, Banerjee A, Slee R, Guenther J, Williams BR, Wiedmer T, Sims PJ, Silverman RH: Phospholipid scramblase 1 potentiates the antiviral activity of interferon. J Virol. 2004 Sep;78(17):8983-93. [Abstract] [Full Text]
  • Product Specific References

  • *Smrz D, Lebduska P, Dráberová L, Korb J, Dráber P: Engagement of Phospholipid Scramblase 1 in Activated Cells: IMPLICATION FOR PHOSPHATIDYLSERINE EXTERNALIZATION AND EXOCYTOSIS. J Biol Chem. 2008 Apr 18;283(16):10904-18. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG2a Isotype Control
  • Example Data

    43-1-13A6
    Fig. 1.

    Fig. 1. Detection of PLSCR1 in rat basophilic leukemia (RBL) cell line lysate (A) and in PLSCR1 immunoprecipitate from RBL lysate (B). The antibody 13A6 (Tec-23) was used both for immunoprecipitation and immunodetection.

     

    scram
    Fig. 2.

    Fig. 2. Immunofluorescence staining of PLSCR1 in rat basophilic leukemia (RBL) cell line using 13A6 (Tec-23) antibody.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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