Exbio antibodies - Rabbit Polyclonal to CD340 / ErbB2 / HER2

categories: Phosphorylation (Kinases, Phosphatases, Phosphotyrosine) (Human), CD and Related Antigens (Human), Cytokine Signaling (Human)

Rabbit Polyclonal to CD340 / ErbB2 / HER2

	Product No.	Form	Quantity	Stock	Price	Datasheet	Shop
	11-493-C050	purified	0.05 mg		choose region

- In stock! Ready for immediate despatch

- Manufacture on order

Background

The oncoprotein ErbB2/HER2 (human epidermal growth factor receptor 2), also known as Neu or CD340, is a 185 kDa transmembrane tyrosine kinase of cell surface growth factor receptor family. It is present in a wide variety cell types of normal human fetal and adult tissues and is frequently overexpressed in human carcinomas (e.g. in 20-30% cases of breast cancer cells). Activation of ErbB2 triggers intracellular signalling events, which are essential for cell growth and differentiation. In the last 20 years ErbB2 antigen has become very important marker and therapy target in patient care.

Entrez Gene: 17q21.1

Specificity

The polyclonal antibody reacts with ErbB2/HER2 oncogen, a 185 kDa transmembrane tyrosine kinase presented in a wide variety cell types of human tissues.

Immunogen

Synthetic peptide corresponding to a site on the extracellular domain of the c-ErbB2 oncoprotein (HER2/neu).

Species Reactivity: Human Negative Species:	Applications: Immunohistochemistry (paraffin sections) Recommended dilution: 10 μg/ml in PBS with 10% FCS and 0.1 % NaN3 Staining technique: standard ABC technique (DAB+) Pretreatment: Fill the staining dishes with 10 mM citrate buffer pH 6.0 (pretreatment solution). Put the staining dishes with the pretreatment solution in the water bath and heat on 95-99°C. Sink the deparaffin sections into the preheated pretreatment solution. Once again heat the water bath and pretreatment solution with sections on 95-99°C - incubate for 40 min in 95-99°C. Remove the dishes with sections from the water bath and keep them cool down for 20 min in RT. Rinse the sections 3 x 5 min in washing buffer (PBS). Incubation: overnight at 4°C Positive tissue: breast carcinoma Application note: Warning: The sections must not drain!! Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	General references Koeppen HK, Wright BD, Burt AD, Quirke P, McNicol AM, Dybdal NO, Sliwkowski MX, Hillan KJ: Overexpression of HER2/neu in solid tumours: an immunohistochemical survey. Histopathology. 2001 Feb;38(2):96-104. [Abstract] Dowsett M, Hanna WM, Kockx M, Penault-Llorca F, Rüschoff J, Gutjahr T, Habben K, van de Vijver MJ: Standardization of HER2 testing: results of an international proficiency-testing ring study. Mod Pathol. 2007 May;20(5):584-91. [Abstract] Hicks DG, Kulkarni S: HER2+ breast cancer: review of biologic relevance and optimal use of diagnostic tools. Am J Clin Pathol. 2008 Feb;129(2):263-73. [Abstract] Yuan W, Wang W, Cui B, Su T, Ge Y, Jiang L, Zhou W, Ning G: Overexpression of ERBB-2 was more frequently detected in malignant than benign pheochromocytomas by multiplex ligation-dependent probe amplification and immunohistochemistry. Endocr Relat Cancer. 2008 Mar;15(1):343-50. [Abstract] [Full Text] Northey JJ, Chmielecki J, Ngan E, Russo C, Annis MG, Muller WJ, Siegel PM: Northey JJ, Chmielecki J, Ngan E, Russo C, Annis MG, Muller WJ, Siegel PM: Signaling through ShcA is required for TGF-{beta} and Neu/ErbB-2 induced breast cancer cell motility and invasion. Mol Cell Biol. 2008 Mar 10 [Abstract] Arora P, Cuevas BD, Russo A, Johnson GL, Trejo J: Persistent transactivation of EGFR and ErbB2/HER2 by protease-activated receptor-1 promotes breast carcinoma cell invasion. Oncogene. 2008 Mar 31 [Abstract]

Species Reactivity:

Human

Negative Species:

Applications:

Immunohistochemistry (paraffin sections)
Recommended dilution: 10 μg/ml in PBS with 10% FCS and 0.1 % NaN3
Staining technique: standard ABC technique (DAB+)
Pretreatment: Fill the staining dishes with 10 mM citrate buffer pH 6.0 (pretreatment solution). Put the staining dishes with the pretreatment solution in the water bath and heat on 95-99°C. Sink the deparaffin sections into the preheated pretreatment solution. Once again heat the water bath and pretreatment solution with sections on 95-99°C - incubate for 40 min in 95-99°C. Remove the dishes with sections from the water bath and keep them cool down for 20 min in RT. Rinse the sections 3 x 5 min in washing buffer (PBS).
Incubation: overnight at 4°C
Positive tissue: breast carcinoma
Application note: Warning: The sections must not drain!!

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

*Koeppen HK, Wright BD, Burt AD, Quirke P, McNicol AM, Dybdal NO, Sliwkowski MX, Hillan KJ: Overexpression of HER2/neu in solid tumours: an immunohistochemical survey. Histopathology. 2001 Feb;38(2):96-104. [Abstract]

*Dowsett M, Hanna WM, Kockx M, Penault-Llorca F, Rüschoff J, Gutjahr T, Habben K, van de Vijver MJ: Standardization of HER2 testing: results of an international proficiency-testing ring study. Mod Pathol. 2007 May;20(5):584-91. [Abstract]

*Hicks DG, Kulkarni S: HER2+ breast cancer: review of biologic relevance and optimal use of diagnostic tools. Am J Clin Pathol. 2008 Feb;129(2):263-73. [Abstract]

*Yuan W, Wang W, Cui B, Su T, Ge Y, Jiang L, Zhou W, Ning G: Overexpression of ERBB-2 was more frequently detected in malignant than benign pheochromocytomas by multiplex ligation-dependent probe amplification and immunohistochemistry. Endocr Relat Cancer. 2008 Mar;15(1):343-50. [Abstract] [Full Text]

*Northey JJ, Chmielecki J, Ngan E, Russo C, Annis MG, Muller WJ, Siegel PM: Northey JJ, Chmielecki J, Ngan E, Russo C, Annis MG, Muller WJ, Siegel PM: Signaling through ShcA is required for TGF-{beta} and Neu/ErbB-2 induced breast cancer cell motility and invasion. Mol Cell Biol. 2008 Mar 10 [Abstract]

*Arora P, Cuevas BD, Russo A, Johnson GL, Trejo J: Persistent transactivation of EGFR and ErbB2/HER2 by protease-activated receptor-1 promotes breast carcinoma cell invasion. Oncogene. 2008 Mar 31 [Abstract]

For research use only. Not for drug, diagnostic or other use.

Example Data

Fig. 1A

Fig. 1B

Fig. 1C

Fig. 1A, 1B, 1C: Immunohistochemistry staining of breast carcinoma (paraffin-embedded sections) with polyclonal anti-human ErbB2/HER2 antibody.
Primary antibody dilution: Fig. 1A 10 μg/ml, Fig. 1B 5 μg/ml, Fig. 1C 2 μg/ml. Detection by Goat anti-rabbit IgG HRP secondary antibody.

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