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Rabbit Polyclonal to Neisseria meningitidis antigen Orf1 / FrpD

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  11-489-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-489-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

Orf1 (FrpD) is a Neisseria meningitidis outer membrane lipoprotein. Subcellular localization and [3H]palmitic acid labeling in Escherichia coli revealed that FrpD is synthesized with a type II signal peptide for export across the cytoplasmic membrane and is, upon processing to a lipoprotein, sorted to the outer bacterial membrane.

Specificity

The polyclonal antibody reacts with Orf1 (FrpD), a 28 - 32 kDa Neisseria meningitidis outer membrane lipoprotein. Orf1 is highly conserved in a set of meningococcal strains representative of all serogroups and does not exhibit any similarity to known sequences of other organisms.

Regulatory Status

Immunogen

Slices containing purified FrpD250-6xHis-tagged protein were excised from 10% SDS-PAGE gels, grinded, mixed with 1 mg/ml of aluminum hydroxide in PBS, and used to immunize a rabbit.

Species Reactivity:

  • Neisseria meningitidis

Negative Species:

Applications:

  • Western Blotting
    Recommended dilution: 0.2 - 1 μg/ml
    Application note: Reducing conditions.
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Prochazkova K, Osicka R, Linhartova I, Halada P, Sulc M, Sebo P: The Neisseria meningitidis outer membrane lipoprotein FrpD binds the RTX protein FrpC. J Biol Chem. 2005 Feb 4;280(5):3251-8. Epub 2004 Nov 2. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Example Data


    Orf1
    Fig. 1. Western Blotting


    Fig. 1. Western Blotting analysis (reducing conditions) of recombinant protein Orf1 in cell lysate of Orf1-transfected E. coli using polyclonal anti-Orf1 antibody.

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