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Mouse Monoclonal to CD2 / LFA-2

LT2 (IgG2b)

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Background

CD2 belongs to T lymphocyte glycoproteins of immunoglobulin superfamily. Its interaction with CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection.

Specificity

The antibody LT2 reacts with CD2, a 50 kDa glycoprotein present on the human peripheral blood T lymphocytes and NK cells; also expressed by all thymocytes.
HLDA VI; WS Code T 6T-008

Regulatory Status

Immunogen

Normal human blood lymphocytes.

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
  • Immunoprecipitation
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Dustin ML: Adhesive bond dynamics in contacts between T lymphocytes and glass-supported planar bilayers reconstituted with the immunoglobulin-related adhesion molecule CD58. J Biol Chem. 1997 Jun 20;272(25):15782-8. [Abstract] [Full Text]
  • *Zhu DM, Dustin ML, Cairo CW, Thatte HS, Golan DE.: Mechanisms of Cellular Avidity Regulation in CD2-CD58-Mediated T Cell Adhesion. ACS Chem Biol. 2006 Nov 21;1(10):649-58. [Abstract]
  • *Pawlowski NN, Struck D, Grollich K, Kuhl AA, Zeitz M, Liesenfeld O, Hoffmann JC.: CD2 deficiency partially prevents small bowel inflammation and improves parasite control in murine Toxoplasma gondii infection. World J Gastroenterol. 2007 Aug 21;13(31):4207-13. [Abstract] [Full Text]
  • Product Specific References

  • Filatov AV, Bachurin PS, Markova NA, Surnakova NE: [A panel of monoclonal antibodies against human lymphocyte antigens] Eksp Onkol. 1989;11(2):28-32. [Abstract]
  • *Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
  • *Gannon GA, Rhind SG, Suzui M, Zamecnik J, Sabiston BH, Shek PN, Shephard RJ: beta-Endorphin and natural killer cell cytolytic activity during prolonged exercise. Is there a connection? Am J Physiol. 1998 Dec;275(6 Pt 2):R1725-34. [Abstract] [Full Text]
  • *Lin CW, Liu TY, Chen SU, Wang KT, Medeiros LJ, Hsu SM: CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features. Blood. 2005 Nov 15;106(10):3567-74. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG2a Isotype Control
  • Example Data


    LT2
    Fig.1. Flow Cytometry analysis


    Fig. 1. Surface staining of human peripheral blood cells with anti-human CD2 (LT2) FITC. Cells in the lymphocyte gate were used for analysis.

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