Mouse Monoclonal to CD11b / Mac-1 alpha
Technical Information Request Inquiry for Bulk Prices
- In stock! Ready for immediate despatch
- Manufacture on order
(integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages.
CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
The antibody MEM-170 recognizes CD11b antigen (Mac-1alpha), a 165-170 kDa transmembrane protein type 1 mainly expressed on monocytes, granulocytes and NK-cells. The antibody blocks (not completely) Mac-1-mediated neutrophil binding to fibrinogen.
HLDA VI., WS Code A054
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.
*Hentzen ER, Neelamegham S, Kansas GS, Benanti JA, McIntire LV, Smith CW, Simon SI. Sequential binding of CD11a/CD18 and CD11b/CD18 defines neutrophil capture and stable adhesion to intercellular adhesion molecule-1. Blood. 2000 Feb 1;95(3):911-20. [Abstract] [Full Text]
*Akramiene D, Kondrotas A, Didziapetriene J, Kevelaitis E.: Effects of beta-glucans on the immune system. Medicina (Kaunas). 2007;43(8):597-606. [Abstract]
*Lawrence PK, Srikumaran S.: CD11b of Ovis canadensis and Ovis aries: molecular cloning and characterization.
Vet Immunol Immunopathol. 2007 Oct 15;119(3-4):287-98.
Product Specific References
*Leukocyte Typing VI. Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
*Machacek C, Supper V, Leksa V, Mitulovic G, Spittler A, Drbal K, Suchanek M, Ohradanova-Repic A, Stockinger H: Folate Receptor β Regulates Integrin CD11b/CD18 Adhesion of a Macrophage Subset to Collagen. J Immunol. 2016 Sep 15;197(6):2229-38. [Abstract] [Full Text]
For research use only. Not for drug, diagnostic or other use.
Mouse IgG1 Isotype Control
Surface staining of human peripheral blood cells with anti-CD11b (MEM-170
) Alexa Fluor® 700.