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Rabbit Polyclonal to NTAL / LAB

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  11-487-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-487-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

NTAL (Non-T cell activation linker), also known as LAB (Linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.

Specificity

The polyclonal antibody reacts with Non-T cell Activation Linker (NTAL), also known as LAB (linker of activated B cells), a 25 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B cells, NK cells and myeloid cells.

Regulatory Status

Immunogen

Recombinant mouse NTAL (aa 30-203).

Species Reactivity:

  • Mouse
  • Rat
  • Other species Not tested

Negative Species:

  • Human

Applications:

  • Western Blotting
    Recommended dilution: 1 μg/ml
    Positive control: mouse spleen
    Sample preparation: Mix lysate with non-reducing SDS-PAGE sample buffer.
    Application note: Non-reducing conditions.
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Horejsí V, Zhang W, Schraven B.: Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat Rev Immunol. 2004 Aug;4(8):603-16. [Abstract]
  • *Yamasaki S, Ishikawa E, Sakuma M, Kanagawa O, Cheng AM, Malissen B, Saito T.: LAT and NTAL mediate immunoglobulin E-induced sustained extracellular signal-regulated kinase activation critical for mast cell survival. Mol Cell Biol. 2007 Jun;27(12):4406-15. [Abstract] [Full Text]
  • Product Specific References

  • *Volná P, Lebduska P, Dráberová L, Símová S, Heneberg P, Boubelík M, Bugajev V, Malissen B, Wilson BS, Horejsí V, Malissen M, Dráber P.: Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL. J Exp Med. 2004 Oct 18;200(8):1001-13. [Abstract] [Full Text]
  • *Heneberg P, Lebduska P, Draberova L, Korb J, Draber P.: Topography of plasma membrane microdomains and its consequences for mast cell signaling. Eur J Immunol. 2006 Oct;36(10):2795-806. [Abstract]
  • *Lebduska P, Korb J, Tůmová M, Heneberg P, Dráber P.: Topography of signaling molecules as detected by electron microscopy on plasma membrane sheets isolated from non-adherent mast cells. J Immunol Methods. 2007 Dec 1;328(1-2):139-51. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse Monoclonal to NTAL / LAB NAP-03 (IgG2a)
  • Rabbit Polyclonal to NTAL / LAB
  • Rabbit Polyclonal to NTAL / LAB
  • Mouse Monoclonal to NTAL / LAB NAP-07 (IgG1)
  • Example Data


    mNTAL polyclonal
    Fig. 1. Western Blotting



    Fig. 1. Western Blotting analysis (non-reducing conditions) of mouse NTAL in mouse spleen tissue.
    Lane 1: immunostaining with polyclonal anti-NTAL antibody
    Lane 2: immunostaining with polyclonal anti-mouse NTAL antibody (specific for N-terminal domain of mouse NTAL; cat. no. 11-486-C100)











    NTAL
    Fig. 2A Distribution of LAT and NTAL on mouse bone marrow-derived mast cells
    NTAL
    Fig. 2B Distribution of LAT and NTAL on rat basophilic leukemia cells


    Fig. 2. Distribution of LAT and NTAL in separate clusters on mast cell plasma membrane sheets.
    LAT and NTAL were detected on mouse bone marrow-derived mast cells (BMMC; 2A) and RBL rat basophilic leukemia cell line (2B) by anti-LAT (LAT-1D1; cat. no. 11-442-C100) and polyclonal anti-NTAL antibody, followed by 5 or 10 nm gold-conjugated secondary antibodies, respectively.



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