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Rabbit Polyclonal to NTAL / LAB

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NTAL (Non-T cell activation linker), also known as LAB (Linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.


The polyclonal antibody recognizes the defined epitope (aa 91-243) of human Non-T cell Activation Linker (NTAL), also known as LAB (linker of activated B cells), a 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B cells, NK cells and myeloid cells.

Regulatory Status


Recombinant cytoplasmic domain (aa 91-243) of human NTAL.

Species Reactivity:

  • Human

Negative Species:

  • Mouse


  • Western Blotting
    Recommended dilution: 1 μg/ml, incubation 60 min in RT; or overnight at 4°C
    Positive control:
    RAMOS human lymphoma cell line
    THP-1 human monocytic leukemia cell line
    Negative control:
    JURKAT human T cell leukemia cell line
    Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.
    Application note: Non-Reducing conditions. 15% separating SDS-PAGE gel.
  • Immunohistochemistry (paraffin sections)
    Recommended dilution:10 μg/ml
    Positive tissue: spleen
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Horejsí V, Zhang W, Schraven B.: Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat Rev Immunol. 2004 Aug;4(8):603-16. [Abstract]
  • *Yamasaki S, Ishikawa E, Sakuma M, Kanagawa O, Cheng AM, Malissen B, Saito T.: LAT and NTAL mediate immunoglobulin E-induced sustained extracellular signal-regulated kinase activation critical for mast cell survival. Mol Cell Biol. 2007 Jun;27(12):4406-15. [Abstract] [Full Text]
  • Product Specific References

  • *Tkaczyk C, Horejsi V, Iwaki S, Draber P, Samelson LE, Satterthwaite AB, Nahm DH, Metcalfe DD, Gilfillan AM.: NTAL phosphorylation is a pivotal link between the signaling cascades leading to human mast cell degranulation following Kit activation and Fc epsilon RI aggregation. Blood. 2004 Jul 1;104(1):207-14. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse Monoclonal to NTAL / LAB NAP-07 (IgG1)
  • Mouse Monoclonal to NTAL / LAB NAP-03 (IgG2a)
  • Rabbit Polyclonal to NTAL / LAB
  • Rabbit Polyclonal to NTAL / LAB
  • Example Data

    Fig. 1.

    Fig. 1. Western Blotting analysis (non-reducing conditions) of NTAL-transfected HEK-293 FT cells using polyclonal anti-human NTAL antibody.
    Lane 1: cell lysate of transfected HEK-293 FT cells
    Lane 2: cell lysate of non-transfected HEK-293 FT cells (negative control)

    (HEK-293 FT is a subclone of the primary human embryonal kidney epithelial cell line.)

    Fig. 2.

    Fig. 2. Immunohistochemistry staining of human spleen (paraffin sections) with anti-NTAL polyclonal.
    Commercially tested by LifeSpan BioSciences.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at
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