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Mouse Monoclonal to CD5

CRIS1 (IgG2a)

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Background

CD5 antigen (T1; 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains.
The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies.
Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (Rheumatoid Arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.

Specificity

The antibody CRIS1 reacts with the cell surface glycoprotein CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes).
HLDA I; WS Code T 29
HLDA III; WS Code T 530

Regulatory Status

Immunogen

stimulated human leukocytes

Species Reactivity:

  • Human
  • Other species Not tested

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution:
    purified: 1 μg/ml
    Positive control:
    Peripheral Blood Lymphocytes (PBL)
    Jurkat human leukemia T-cell line
    HPB human leukemia T-cell line
    MOLT-4 human leukemia T-cell line
  • Immunoprecipitation
  • Western Blotting
    Recommended dilution: 1-2 μg/ml
    Positive control:
    Jurkat human leukemia T-cell line
    HPB human leukemia T-cell line
  • Immunohistochemistry (frozen sections)
  • ELISA
    The antibody CRIS1 can be used in the Sandwich ELISA as the detection antibody in pair with the capture antibody MEM-32
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Freedman AS, Freeman G, Whitman J, Segil J, Daley J, Levine H, Nadler LM: Expression and regulation of CD5 on in vitro activated human B cells. Eur J Immunol. 1989 May;19(5):849-55. [Abstract]
  • Raman C: CD5, an important regulator of lymphocyte selection and immune tolerance. Immunol Res. 2002;26(1-3):255-63. [Abstract]
  • Product Specific References

  • *Leukocyte Typing III., McMichael A. J. et al. (Eds.), Oxford University Press (1987).
  • *Arrizabalaga P, Mirapeix E, Darnell A, Torras A, Revert L: Cellular immunity analysis using monoclonal antibodies in human glomerulonephritis. Nephron. 1989;53(1):41-9. [Abstract]
  • *Alberola-Ila J, Places L, Cantrell DA, Vives J, Lozano F: Intracellular events involved in CD5-induced human T cell activation and proliferation. J Immunol. 1992 Mar 1;148(5):1287-93. [Abstract]
  • *Guarne A, Bravo J, Calvo J, Lozano F, Vives J, Fita I: Conformation of the hypervariable region L3 without the key proline residue. Protein Sci. 1996 Jan;5(1):167-9. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse Monoclonal to CD5 MEM-32 (IgG1)
  • Mouse IgG2a Isotype Control
  • Example Data


    CRIS1
    Fig. 1a FC analysis - Histogram
    CRIS1




    Fig. 1b FC analysis - Dot plot


    Fig. 1. Flow Cytometry analysis of human Peripheral Blood Lymphocytes (PBL) stained with CRIS1 antibody (dilution of purified antibody 1 μg/ml); Fig. 1a Histogram - gated on lymphocytes, overlay with isotypic control (mouse IgG2a); Fig. 1b Dot plot.

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