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Mouse Monoclonal to CD66e / CEA

CB30 (IgG1)

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The CD66e (CEA; 180-200 kDa) is a member of carcinoembryonic antigens, immunoglobulin supergene family and consists of a single N domain (structural homology to the immunoglobulin variable) and six immunoglobulin constant-like A (A1, A2, A3) and B domains (B1, B2, B3). Human CD66e is heavily glycosylated GPI anchored protein capable of both homophilic and heterophilic adhesion.
Disease relevance: The CD66e may play a role in the process of metastasis of cancer cells. CD66e is found in serum and it is clinically used as a tumor marker for early detection of disease due to its expression in adenocarcinomas - potential target of tumor imaging and drug targeting.


The antibody CB30 recognizes CD66e (CEA; 180-200 kDa), a cell surface bound carcinoembryonic antigen mainly expressed on epithelial cells.

Regulatory Status


Human carcinoembryonic antigen (CEA; CEACAM5)

Species Reactivity:

  • Human

Negative Species:


  • Flow Cytometry
  • Immunoprecipitation
  • Immunohistochemistry (paraffin sections)
    Staining technique: Standard ABC technique (DAB+)
    Recommended dilution: 10 μg/ml (1:100)
    Positive tissue: Adenocarcinoma of colon
    Pretreatment: 0,1% pepsin in 0,1 M HCl for 30 min at room temperature
  • Immunohistochemistry (frozen sections)
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Gangopadhyay A, Bajenova O, Kelly TM, Thomas P: Carcinoembryonic antigen induces cytokine expression in Kuppfer cells: implications for hepatic metastasis from colorectal cancer. Cancer Res. 1996 Oct 15;56(20):4805-10. [Abstract]
  • *Thompson JA, Grunert F, Zimmermann W: Carcinoembryonic antigen gene family: molecular biology and clinical perspectives. J Clin Lab Anal. 1991;5(5):344-66. Review. [Abstract]
  • *Oikawa S, Nakazato H, Kosaki G: Primary structure of human carcinoembryonic antigen (CEA) deduced from cDNA sequence. Biochem Biophys Res Commun. 1987 Jan 30;142(2):511-8. [Abstract] [Full Text]
  • *Chen CJ, Li LJ, Maruya A, Shively JE: In vitro and in vivo footprint analysis of the promoter of carcinoembryonic antigen in colon carcinoma cells: effects of interferon gamma treatment. Cancer Res. 1995 Sep 1;55(17):3873-82. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data

    IHC (P) of CB30
    Fig. 1A Immunohistochemistry of paraffin-embedded sections (colorectal carcinoma)
    IHC (P) of CB30
    Fig. 1B Immunohistochemistry of paraffin-embedded sections (colorectal carcinoma)

    Fig. 1A, 1B: Immunohistochemistry staining of colorectal carcinoma (paraffin-embedded sections) with anti-human CD66e (CB30).
    Primary antibody dilution: 10 μg/ml.

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