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Mouse Monoclonal to Cytokeratin 10+13

DE-K13 (IgG2a)

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Background

Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Cytokeratins 10 and 13 both belong to type I family (acidic cytokeratins).

Specificity

The antibody DE-K13 reacts with Cytokeratin 10 (56.5 kDa) and Cytokeratin 13 (53 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. DE-K13 recognizes only Cytokeratin 13 on formalin-fixed, paraffin-embedded tissue sections.

Regulatory Status

Immunogen

Cytoskeletal preparation extracted from human ectocervical epithelium.

Species Reactivity:

  • Human
  • Feline (Cat)
  • Other species Not tested

Negative Species:

Applications:

  • Western Blotting
    Recommended dilution: 1-3 μg/ml
  • Immunohistochemistry (paraffin sections)
    Staining technique: Standard ABC technique (DAB+).
    Recommended dilution: 10 μg/ml (1:100)
    Positive tissue: tonsil
    Pretreatment: 0.1% pepsin in 0.1 M HCl for 30 min at room temperature; or Target Retrieval Solution - High pH
    Application note: The antibody DE-K13 reacts exclusively with Cytokeratin 13.
  • Immunohistochemistry (frozen sections)
    Recommended dilution: Use at an assay dependent dilution.
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Ivanyi D, Minke JM, Hageman C, Groeneveld E, van Doornewaard G: Patterns of expression of feline cytokeratins in healthy epithelia and mammary carcinoma cells. Am J Vet Res. 1992 Mar;53(3):304-14. [Abstract]
  • *Ivanyi D, Minke JM, Hageman C, Groeneveld E, van Doornewaard G, Misdorp W: Cytokeratins as markers of initial stages of squamous metaplasia in feline mammary carcinomas. Am J Vet Res. 1993 Jul;54(7):1095-102. [Abstract]
  • *Kozaki M, Nakamura Y, Iguchi M, Kano R, Watanabe S, Fujiwara K, Hasegawa A: Immunohistochemical analysis of cytokeratin expression in dog skin. J Vet Med Sci. 2001 Jan;63(1):1-4. [Abstract] [Full Text]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse Monoclonal to Cytokeratin 10 VIK-10 (IgG1)
  • Mouse IgG2a Isotype Control
  • Example Data



    DE-K13
    Fig. 1. Immunohistochemistry on paraffin-embedded sections (tonsil)


    Fig. 1. Immunohistochemistry staining of tonsil (paraffin-embedded sections) with anti-Cytokeratin 10+13, (DE-K13).
    The antibody DE-K13 stains only Cytokeratin 13 when used on formalin-fixed, paraffin-embedded tissue sections.





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