Mouse Monoclonal to GST / Glutathione-S-Transferase, GST tag
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The GST (Glutathione-S-Transferase) is a 26kDa protein encoded by the parasite Schistosoma japonicum, easily purified by affinity chromatography on immobilized glutathione. GST is utilized as a fusion protein with foreign proteins in a range of prokaryotic expression vectors, including the pGEX family of vectors.
Antibodies against GST will detect the expressed GST protein or a GST-fusion protein, and can be utilized for immuno-purification of GST-fusion proteins.
The antibody S-tag-05 is a mouse monoclonal antibody with high affinity to the glutathione-S-transferase (GST) from Schistosoma japonicum. This purified antibody
is suitable for detecting fusion proteins
containing the GST-Tag sequence expressed
in E. coli, yeast, mammalian, and
in vitro transcription/translation systems.
GST fusion protein
- Recognizes fusion proteins in all species
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.
For research use only. Not for drug, diagnostic or other use.
Mouse IgG2b Isotype Control
Fig. 1. Isolation of GST
Isolation of Glutathione-S-Transferase (GST) overexpressed in E. coli
Western blot was immunostained by anti-GST (S-tag-05
affinity purification of GST from bacterial cell lysate using commercial sorbent Glutathione-SepharoseLane 2:
affinity purification of GST from bacterial cell lysate using immunosorbent prepared from anti-GST (S-tag-02; cat. no. 11-424-C100)