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Mouse Monoclonal to GST / Glutathione-S-Transferase, GST tag

S-tag-05 (IgG2b)

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Background

The GST (Glutathione-S-Transferase) is a 26kDa protein encoded by the parasite Schistosoma japonicum, easily purified by affinity chromatography on immobilized glutathione. GST is utilized as a fusion protein with foreign proteins in a range of prokaryotic expression vectors, including the pGEX family of vectors.

Antibodies against GST will detect the expressed GST protein or a GST-fusion protein, and can be utilized for immuno-purification of GST-fusion proteins.

Specificity

The antibody S-tag-05 is a mouse monoclonal antibody with high affinity to the glutathione-S-transferase (GST) from Schistosoma japonicum. This purified antibody is suitable for detecting fusion proteins containing the GST-Tag sequence expressed in E. coli, yeast, mammalian, and in vitro transcription/translation systems.

Regulatory Status

Immunogen

GST fusion protein

Species Reactivity:

  • Recognizes fusion proteins in all species

Negative Species:

Applications:

  • Western Blotting
    Recommended dilution: 1 μg/ml
    Positive control: GST fusion protein
    Application note: Reducing conditions
  • Immunoprecipitation
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

References

  • Unpublished.
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG2b Isotype Control
  • Example Data


    S-tag-05
    Fig. 1. Isolation of GST


    Fig. 1. Isolation of Glutathione-S-Transferase (GST) overexpressed in E. coli.
    Western blot was immunostained by anti-GST (S-tag-05).
    Lane 1: affinity purification of GST from bacterial cell lysate using commercial sorbent Glutathione-Sepharose
    Lane 2: affinity purification of GST from bacterial cell lysate using immunosorbent prepared from anti-GST (S-tag-02; cat. no. 11-424-C100)

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