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Mouse Monoclonal to HLA-DR

MEM-12 (IgG1)

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  11-474-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
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  PB-474-T025 Pacific Blue™ 25 tests yes choose region PDF datasheetHTML datasheet buy
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  1F-474-T025 FITC 25 tests yes choose region PDF datasheetHTML datasheet buy
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Background

HLA-DR, a member of MHC class II glycoproteins, that bind intracellularly processed peptides and present them to the Th cells, is composed of 36 kDa alpha chain and 27 kDa beta chain, both anchored in the plasma membrane. Together with other MHC II molecules HLA-DR plays a central role in the immune system.

Specificity

The antibody MEM-12 recognizes common epitope on human HLA-DR which is dependent on the association of alpha and beta chains. DR is the isotype of human MHC Class II molecules expressed on antigen-presenting cells (APC; dendritic cells, B lymphocytes, monocytes, macrophages).

Regulatory Status

Immunogen

thymocyte membrane

Species Reactivity:

  • Human

Negative Species:

Applications:

  • Flow Cytometry
    Recommended dilution:2 μg/ml
    Positive control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line
  • Immunoprecipitation
    Positive control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line
  • Western Blotting
    Positive control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line
    Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Do not heat/boil.
    Application note: Non-reducing conditions.
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

  • *Horejsi V, Nemec M, Angelisova P, Kristofova H, Gorga JC, Hilgert I: Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens. Tissue Antigens. 1986 Nov;28(5):288-97. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Example Data


    MEM-12


    Fig. 1. Surface staining of human peripheral blood cells with anti-human HLA-DR (MEM-12) FITC.


    MEM-12 APC


    Fig. 2. Surface staining of human peripheral blood cells with anti-human HLA-DR (MEM-12) APC.


    mem-12 PE


    Fig. 3. Surface staining of human peripheral blood cells with anti-human HLA-DR (MEM-12) PE.


    MEM-12 PB


    Fig. 4. Surface staining of human peripheral blood cells with anti-human HLA-DR (MEM-12) Pacific BlueTM.


    MEM-12 PC


    Fig. 5. Surface staining of human peripheral blood cells with anti-human HLA-DR (MEM-12) PerCP.


    MEM-12 AF700


    Fig. 6. Surface staining of human peripheral blood cells with anti-human HLA-DR (MEM-12) Alexa Fluor® 700.


    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at www.exbio.cz.
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