categories: MHC (Major Histocompatibility Complex) and Related Antigens (Human)

Mouse Monoclonal to HLA-DR

MEM-12 (IgG1)

Product No.	Form	Quantity	Price
11-474-C025	purified	0.025 mg	choose region
11-474-C100	purified	0.1 mg	choose region
PB-474-T025	Pacific Blue™	25 tests	choose region
PB-474-T100	Pacific Blue™	100 tests	choose region
1F-474-T025	FITC	25 tests	choose region
1F-474-T100	FITC	100 tests	choose region
A4-474-T025	Alexa Fluor® 488	25 tests	choose region
A4-474-T100	Alexa Fluor® 488	100 tests	choose region
1P-474-T025	PE	25 tests	choose region
1P-474-T100	PE	100 tests	choose region
1A-474-T025	APC	25 tests	choose region
1A-474-T100	APC	100 tests	choose region
PC-474-T025	PerCP	25 tests	choose region
PC-474-T100	PerCP	100 tests	choose region
A7-474-T025	Alexa Fluor® 700	25 tests	choose region
A7-474-T100	Alexa Fluor® 700	100 tests	choose region

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Background

HLA-DR, a member of MHC class II glycoproteins, that bind intracellularly processed peptides and present them to the Th cells, is composed of 36 kDa alpha chain and 27 kDa beta chain, both anchored in the plasma membrane. Together with other MHC II molecules HLA-DR plays a central role in the immune system.

Specificity

The antibody MEM-12 recognizes common epitope on human HLA-DR which is dependent on the association of alpha and beta chains. DR is the isotype of human MHC Class II molecules expressed on antigen-presenting cells (APC; dendritic cells, B lymphocytes, monocytes, macrophages).

Immunogen

thymocyte membrane

Species Reactivity: Human Negative Species:	Applications: Flow Cytometry Recommended dilution:2 μg/ml Positive control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line Immunoprecipitation Positive control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line Western Blotting Positive control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Do not heat/boil. Application note: Non-reducing conditions. Usage note: Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.	Product Specific References *Horejsi V, Nemec M, Angelisova P, Kristofova H, Gorga JC, Hilgert I: Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens. Tissue Antigens. 1986 Nov;28(5):288-97. [Abstract]

Species Reactivity:

Human

Negative Species:

Applications:

Flow Cytometry
Recommended dilution:2 μg/ml
Positive control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line
Immunoprecipitation
Positive control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line
Western Blotting
Positive control: B-lymphocytes, Monocytes, DAUDI cell line, RAJI cell line
Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Do not heat/boil.
Application note: Non-reducing conditions.

Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

Product Specific References

*Horejsi V, Nemec M, Angelisova P, Kristofova H, Gorga JC, Hilgert I: Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens. Tissue Antigens. 1986 Nov;28(5):288-97. [Abstract]

For research use only. Not for drug, diagnostic or other use.

Related Products

Mouse IgG1 Isotype Control

Example Data

Fig. 1. Flow Cytometry analysis

Fig. 1. Surface staining of human peripheral blood cells with anti-human HLA-DR (MEM-12) FITC.

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