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Mouse Monoclonal to MAP2ab

MT-08 (IgG1)

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Product No. Form Quantity Stock Price Datasheet Shop
  11-473-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-473-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
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Background

MAP2a and 2b (270 kDa) being found mostly in dendrites, stabilize microtubules (shift the reaction kinetics in addition of new subunits and microtubule growth) and participate in determining the structure of different parts of vertebrate nerve cells.

Specificity

The antibody MT-08 recognizes an epitope (aa 1375-1395) located in central domain of molecule Microtubule Associated Protein 2ab (MAP2ab).

Regulatory Status

Immunogen

Microtubule protein (bovine brain) enriched for kinesin

Species Reactivity:

  • Human
  • Porcine
  • Mouse

Negative Species:

Applications:

  • Immunoprecipitation
    Positive material: Porcine brain
  • Western Blotting
    Positive control:
    Porcine brain
    Microtubule associated protein 2 (MAP2)
  • Immunohistochemistry (paraffin sections)
    Recommended dilution: 10 μg/ml
    Positive tissue: brain
  • Immunohistochemistry (frozen sections)
    Positive tissue: Mouse brain
  • Immunocytochemistry
    Positive control: human neuroblastoma SH-SY5Y
  • ELISA
    Positive control:
    Porcine brain
    Microtubule associated protein 2 (MAP2)
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

References

  • Unpublished.
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgG1 Isotype Control
  • Mouse Monoclonal to MAP2ab MT-07 (IgG1)
  • Mouse Monoclonal to MAP2ab MT-01 (IgG1)
  • Example Data


    MT-08
    Fig. 1. Western Blotting


    Fig. 1. Western Blotting analysis (reducing conditions) of microtubules partially purified from porcine brain lysate.
    Lane 1: immunostaining with anti-MAP2ab (MT-01; cat. no. 11-253-C100)
    Lane 2: immunostaining with anti-MAP2ab (MT-07; cat. no. 11-472-C100)
    Lane 3: immunostaining with anti-MAP2ab (MT-08)

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