System Certification ISO 9001:2000
Exbio Antibodies CZ / SK eShop
by categories | a-z list | Application type: Flow Cytometry | Immunohistochemistry | Functional Studies

Mouse Monoclonal to beta-tubulin / TUBB

TU-13 (IgM)

Technical Information Request    Inquiry for Bulk Prices

Product No. Form Quantity Stock Price Datasheet Shop
  11-444-C025 purified 0.025 mg yes choose region PDF datasheetHTML datasheet buy
  11-444-C100 purified 0.1 mg yes choose region PDF datasheetHTML datasheet buy
- In stock! Ready for immediate despatch - Manufacture on order


The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening – this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains.

The beta-tubulin (relative molecular weight around 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain.


The antibody TU-13 recognizes an epitope on N-terminal structural domain of beta-tubulin in various species.

Regulatory Status


beta-tubulin from porcine brain

Species Reactivity:

  • Human
  • Porcine
  • Mouse
  • Plants

Negative Species:


  • Western Blotting
    Recommended dilution: 1 μg/ml
    Positive control: RAJI human cell line, RAMOS human cell line, THP-1 human cell line, HeLa human cell line, EL4 mouse cell line, MCF7 human cell line
    Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Boil for 5 min.
    Application note: Reducing conditions. Sonication of MCF7 cells is recommended.
  • Immunohistochemistry (frozen sections)
  • Immunocytochemistry
Usage note:
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.

General references

  • *Linhartova I, Draberova E, Viklicky V, Draber P: Distribution of non-class-III beta-tubulin isoforms in neuronal and non-neuronal cells. FEBS Lett. 1993 Mar 29;320(1):79-82. [Abstract] [Full Text]
  • *Lewis, S.A., Cowan, N.J.: Tubulin genes: structure, expression, and regulation. In: Avila, J. (ed.): Microtubule proteins. Boca Raton: CRC Press, Inc. 1990. Pp. 37-66.
  • Product Specific References

  • *Draber P, Lagunowich LA, Draberova E, Viklicky V, Damjanov I: Heterogeneity of tubulin epitopes in mouse fetal tissues. Histochemistry. 1988;89(5):485-92. [Abstract]
  • *Draber P, Vater W, Bohm KJ, Kuklova E, Unger E: Inhibition of microtubule assembly in vitro by anti-tubulin monoclonal antibodies. FEBS Lett. 1990 Mar 26;262(2):209-11. [Abstract]
  • For research use only. Not for drug, diagnostic or other use.

    Related Products

  • Mouse IgM Isotype Control
  • Example Data

    TU-13 pur WB

    Fig. 1. Western blotting analysis of porcine brain lysate using anti-beta tubulin (TU-13) purified.

    Unless indicated otherwise, all products are For Research Use Only and not for diagnostic or therapeutic use. Not for resale or transfer either as a stand-alone product or as a component of another product without written consent of EXBIO. EXBIO will not be held responsible for patent infringement or other violations that may occur with the use of our products. All orders are accepted subject to EXBIO´s term and conditions which are available at
    © 2003 EXBIO. All rights reserved.
    terms and conditions | privacy policy