- Flow Cytometry
Recommended dilution: 1 μg/ml
- Western Blotting
- Functional Application
The bound of antibody 1aG4/C5 to the transfected mast cells induces the activation processes which are similar, but not identical, to the antigen activation of IgE stimulated cells.
Other studies approve that the antibody 1aG4/C5 precipitates complexes with Lyn protein tyrosine kinase.
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.
*Haeryfar SM, Hoskin DW: Thy-1: more than a mouse pan-T cell marker. J Immunol. 2004 Sep 15;173(6):3581-8. [Abstract] [Full Text]
*Rege TA, Hagood JS: Thy-1, a versatile modulator of signaling affecting cellular adhesion, proliferation, survival, and cytokine/growth factor responses. Biochim Biophys Acta. 2006 Oct;1763(10):991-9. [Abstract] [Full Text]
*Rege TA, Hagood JS: Thy-1 as a regulator of cell-cell and cell-matrix interactions in axon regeneration, apoptosis, adhesion, migration, cancer, and fibrosis. FASEB J. 2006 Jun;20(8):1045-54. [Abstract] [Full Text]
Product Specific References
*Draber P, Zikan J, Vogtiskova M: Establishment and characterization of permanent murine hybridomas secreting monoclonal anti-thy-1 antibodies. J Immunogenet. 1980 Dec;7(6):455-74.
*Zikan J, Draber P, Vojtiskova M: Monoclonal IgG3 (kappa) antibodies against murine Thy-1.2 antigen produced by murine hybridomas. Differences in the specificity of the antigen binding site and in the structure of the hinge region. Folia Biol (Praha). 1982;28(6):377-94.
*Draberova L, Draber P: Thy-1 glycoprotein and src-like protein-tyrosine kinase p53/p56lyn are associated in large detergent-resistant complexes in rat basophilic leukemia
cells. Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3611-5.
*Bradley JE, Ramirez G, Hagood JS: Roles and regulation of Thy-1, a context-dependent modulator of cell phenotype. Biofactors. 2009 May-Jun;35(3):258-65. [Abstract]
*Heneberg P, Lebduska P, Draberova L, Korb J, Draber P: Topography of plasma membrane microdomains and its consequences for mast cell signaling. Eur J Immunol. 2006 Oct;36(10):2795-806.