|11-465-C025||purified||0.025 mg||choose region|
|11-465-C100||purified||0.1 mg||choose region|
|A6-465-C025||Alexa Fluor® 647||0.025 mg||choose region|
|A6-465-C100||Alexa Fluor® 647||0.1 mg||choose region|
Indicated dilutions are recommended starting points for use of this product. Working concentrations should be determined by the investigator.
Product Specific References
Fig. 2. Immunofluorescence staining of mouse fibroblasts using anti-gamma-tubulin (TU-30; direct conjugate with Dyomics 547, red). Nuclei were stained with DAPI (blue).
Fig. 3. Immunofluorescence staining of microtubular networks in 3T3 mouse fibroblasts.
3A – metaphase; 3B – anaphase; 3C - telophase
Gamma-tubulin (red) stained with anti-gamma-tubulin (TU-30), alpha-tubulin (green) with polyclonal anti-alpha-tubulin antibody and nuclei with DAPI (blue).
Fig. 4. Differential reactivity of monoclonal antibodies to γ-tubulin with human γ-tubulin isotypes. (A) Immunoblots of total cell lysates from SH-SY5Y cells, expressing TagRFP-tagged human γ-tubulin 1 (γ-Tb1) or γ-tubulin 2 (γ-Tb2), probed with Abs to γ-tubulin (TU-30, TU-32), TagRFP (RFP) and GAPDH. In control samples, only secondary anti-mouse Ab was applied. (B) Immunoblots of immobilized GST-tagged human C-terminal regions (a.a. 362-451) of γ-Tb1 or γ-Tb2 probed with Abs to γ-tubulin (TU-30, TU-32) and GST. In control samples, only secondary anti-mouse Ab was applied.